Crystallization (Comment) | Organism |
---|---|
the aspartate product in the crystal structure of L-ASP exists in an unusual alpha-COOH protonation state. The crystal structures may represent intermediate steps rather than initial binding. The substrate's alpha-carboxyl may serve as a proton acceptor and activate one of the catalytic threonines during L-ASP's nucleophilic attack on the amide carbon | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
L-asparagine | K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C | Escherichia coli | |
0.021 | - |
L-asparagine | K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C | Escherichia coli | |
0.025 | - |
L-asparagine | K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C | Escherichia coli | |
0.068 | - |
L-asparagine | K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C | Escherichia coli | |
1.6 | - |
L-glutamine | K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00805 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | reaction of EC 3.5.1.2 | Escherichia coli | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AnsB | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
L-glutamine | pH 8.0, 37°C | Escherichia coli | |
48 | - |
L-asparagine | presence of 8 mM Gln, pH 8.0, 37°C | Escherichia coli | |
60 | - |
L-asparagine | pH 8.0, 37°C | Escherichia coli | |
67 | - |
L-asparagine | presence of 0.9 mM Gln, pH 8.0, 37°C | Escherichia coli | |
72 | - |
L-asparagine | presence of 0.3 mM Gln, pH 8.0, 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
710 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C | Escherichia coli | |
1400 | - |
L-glutamine | using K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C | Escherichia coli | |
2700 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C | Escherichia coli | |
3300 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C | Escherichia coli | |
3400 | - |
L-asparagine | using K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C | Escherichia coli |