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Literature summary for 3.5.1.1 extracted from

  • Anishkin, A.; Vanegas, J.M.; Rogers, D.M.; Lorenzi, P.L.; Chan, W.K.; Purwaha, P.; Weinstein, J.N.; Sukharev, S.; Rempe, S.B.
    Catalytic role of the substrate defines specificity of therapeutic L-asparaginase (2015), J. Mol. Biol., 427, 2867-2885 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
the aspartate product in the crystal structure of L-ASP exists in an unusual alpha-COOH protonation state. The crystal structures may represent intermediate steps rather than initial binding. The substrate's alpha-carboxyl may serve as a proton acceptor and activate one of the catalytic threonines during L-ASP's nucleophilic attack on the amide carbon Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
L-asparagine K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C Escherichia coli
0.021
-
L-asparagine K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C Escherichia coli
0.025
-
L-asparagine K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C Escherichia coli
0.068
-
L-asparagine K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C Escherichia coli
1.6
-
L-glutamine K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00805
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
Escherichia coli L-aspartate + NH3
-
?
L-glutamine + H2O reaction of EC 3.5.1.2 Escherichia coli L-glutamate + NH3
-
?

Synonyms

Synonyms Comment Organism
AnsB
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
L-glutamine pH 8.0, 37°C Escherichia coli
48
-
L-asparagine presence of 8 mM Gln, pH 8.0, 37°C Escherichia coli
60
-
L-asparagine pH 8.0, 37°C Escherichia coli
67
-
L-asparagine presence of 0.9 mM Gln, pH 8.0, 37°C Escherichia coli
72
-
L-asparagine presence of 0.3 mM Gln, pH 8.0, 37°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
710
-
L-asparagine using K0.5 value, Hill coefficient 1.0, presence of 8 mM Gln, pH 8.0, 37°C Escherichia coli
1400
-
L-glutamine using K0.5 value, Hill coefficient 1.1, pH 8.0, 37°C Escherichia coli
2700
-
L-asparagine using K0.5 value, Hill coefficient 1.4, presence of 0.9 mM Gln, pH 8.0, 37°C Escherichia coli
3300
-
L-asparagine using K0.5 value, Hill coefficient 1.5, pH 8.0, 37°C Escherichia coli
3400
-
L-asparagine using K0.5 value, Hill coefficient 1.4, presence of 0.3 mM Gln, pH 8.0, 37°C Escherichia coli