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Literature summary for 3.5.1.1 extracted from
- Isolation, purification and characterization of an extracellular L-asparaginase produced by a newly isolated Bacillus megaterium strain MG1 from the water bodies of Moraghat forest, Jalpaiguri, India (2019), J. Gen. Appl. Microbiol., 65, 137-144 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, 16.5% of initial activity | Priestia megaterium |  |
| Co2+ | 10 mM, 35.6% of initial activity | Priestia megaterium | |
| Cu2+ | 10 mM, 22.3% of initial activity | Priestia megaterium | |
| Fe2+ | 10 mM, 13.3% of initial activity | Priestia megaterium | |
| Hg2+ | 10 mM, 23% of initial activity | Priestia megaterium | |
| Mg2+ | 10 mM, 3.3% of initial activity | Priestia megaterium | |
| Mn2+ | 10 mM, 20.5% of initial activity | Priestia megaterium | |
| SDS | 2 mM, 3.1% of initial activity | Priestia megaterium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
L-asparagine | 37°C, pH 8.5 | Priestia megaterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | 10 mM, 110% of initial activity | Priestia megaterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 185000 | - |
gel filtration | Priestia megaterium |
| 185000 | - |
PAGE | Priestia megaterium |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| DMSO | 50% , 78% residual activity | Priestia megaterium |
| Ethanol | 50% , 79% residual activity | Priestia megaterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
- |
- |
| Priestia megaterium MG1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Priestia megaterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 215 | - |
37°C, pH 8.5 | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Priestia megaterium | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Priestia megaterium MG1 | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 47000, SDS-PAGE | Priestia megaterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Priestia megaterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | more than 40% of maximum activity within | Priestia megaterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, at least 40% residual activity | Priestia megaterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Priestia megaterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | more than 50% of maximum activity within | Priestia megaterium |
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