Application | Comment | Organism |
---|---|---|
analysis | assembly a microplate-based biosensor for the determination of L-asparagine in biological samples. The enzyme is immobilized by crosslinking with glutaraldehyde in a microplate in 96-well format. The sensing is based on the colorimetric measurement of ammonia formation using the Nessler's reagent. The sensor enables monitoring of L-asparagine levels in serum and foods samples in the concentration range 10-200 microM, with a detection limit of 10 microM for L-asparagine | Pectobacterium carotovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
0.3 | - |
succinamic acid | pH 8.0, 25°C | Pectobacterium carotovorum | |
0.5 | - |
Nalpha-acetyl-L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
5.2 | - |
L-glutamine | pH 8.0, 25°C | Pectobacterium carotovorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
single-step procedure | Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | reaction of EC 3.5.1.2 | Pectobacterium carotovorum | L-glutamate + NH3 | - |
? | |
additional information | no substrate: D-asparagine, beta-alanine amide | Pectobacterium carotovorum | ? | - |
? | |
Nalpha-acetyl-L-asparagine + H2O | - |
Pectobacterium carotovorum | Nalpha-acetyl-L-aspartate + NH3 | - |
? | |
succinamic acid + H2O | - |
Pectobacterium carotovorum | succinate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
L-glutamine | pH 8.0, 25°C | Pectobacterium carotovorum | |
4.1 | - |
succinamic acid | pH 8.0, 25°C | Pectobacterium carotovorum | |
4.1 | - |
Nalpha-acetyl-L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
55.2 | - |
L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
L-glutamine | pH 8.0, 25°C | Pectobacterium carotovorum | |
8.2 | - |
Nalpha-acetyl-L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum | |
13.7 | - |
succinamic acid | pH 8.0, 25°C | Pectobacterium carotovorum | |
788.6 | - |
L-asparagine | pH 8.0, 25°C | Pectobacterium carotovorum |