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Literature summary for 3.5.1.1 extracted from
- PEG-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant L-asparaginase from Erwinia carotovora (2015), Biochemistry (Moscow), 80, 113-119 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | method based on IR spectroscopy to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates. The method is reagentx02free and allows fast and reliable determination of parameters | Pectobacterium carotovorum |
| synthesis | conjugation of enzyme to PEG-chitosan and glycol-chitosan imoproves catalytic efficiency 3- to 6fold under physiological conditions and enhances its resistance to thermal inactivation | Pectobacterium carotovorum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pectobacterium carotovorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pectobacterium carotovorum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? |  |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
native enzyme, 50 min, complete loss of activity. Enzyme conjugated to glycol-chitosan, 50 min, 40% residual activity | Pectobacterium carotovorum |
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