Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.1 extracted from

  • Zuo, S.; Xue, D.; Zhang, T.; Jiang, B.; Mu, W.
    Biochemical characterization of an extremely thermostable l-asparaginase from Thermococcus gammatolerans EJ3 (2014), J. Mol. Catal. B, 109, 122-129.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermococcus gammatolerans

General Stability

General Stability Organism
after incubation with various concentration of urea for 6 h, the purified enzyme samples almost do not exhibit any change in enzyme activity even by incubation with 8 M of urea Thermococcus gammatolerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
L-asparagine at pH 8.5 and 85°C Thermococcus gammatolerans
24.1
-
D-asparagine at pH 8.5 and 85°C Thermococcus gammatolerans
39.5
-
L-glutamine at pH 8.5 and 85°C Thermococcus gammatolerans
77.6
-
D-glutamine at pH 8.5 and 85°C Thermococcus gammatolerans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ marginal increase in enzyme activity in the presence of 1 mM Mg2+ (less than 15%) Thermococcus gammatolerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Thermococcus gammatolerans
36500
-
2 * 36500, predicted from amino acid sequence Thermococcus gammatolerans
74000
-
gel filtration Thermococcus gammatolerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Thermococcus gammatolerans best substrate L-aspartate + NH3
-
?
L-asparagine + H2O Thermococcus gammatolerans EJ3 best substrate L-aspartate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus gammatolerans C5A6Q4
-
-
Thermococcus gammatolerans EJ3 C5A6Q4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Thermococcus gammatolerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.925
-
with D-glutamine as substrate, at pH 8.5 and 85°C Thermococcus gammatolerans
2.926
-
with L-glutamine as substrate, at pH 8.5 and 85°C Thermococcus gammatolerans
3.753
-
with D-asparagine as substrate, at pH 8.5 and 85°C Thermococcus gammatolerans
7.622
-
with L-asparagine as substrate, at pH 8.5 and 85°C Thermococcus gammatolerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O
-
Thermococcus gammatolerans D-aspartate + NH3
-
?
D-asparagine + H2O
-
Thermococcus gammatolerans EJ3 D-aspartate + NH3
-
?
D-glutamine + H2O
-
Thermococcus gammatolerans D-glutamate + NH3
-
?
D-glutamine + H2O
-
Thermococcus gammatolerans EJ3 D-glutamate + NH3
-
?
L-asparagine + H2O best substrate Thermococcus gammatolerans L-aspartate + NH3
-
?
L-asparagine + H2O best substrate Thermococcus gammatolerans EJ3 L-aspartate + NH3
-
?
L-glutamine + H2O
-
Thermococcus gammatolerans L-glutamate + NH3
-
?
L-glutamine + H2O
-
Thermococcus gammatolerans EJ3 L-glutamate + NH3
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Thermococcus gammatolerans
homodimer 2 * 36500, predicted from amino acid sequence Thermococcus gammatolerans

Synonyms

Synonyms Comment Organism
L-asparaginase
-
Thermococcus gammatolerans
L-asparagine amidohydrolase
-
Thermococcus gammatolerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermococcus gammatolerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 the enzyme shows more than 75% of relative activity in the range of 70-95°C Thermococcus gammatolerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 95 the enzyme retains more than 60% of its initial activityafter 30 min at 70–95°C, and retains 66%, 60%, 54%, 53%, and 43% of its initial activity after 2 h of exposure at 70, 80, 85, 90, and 95°C, respectively Thermococcus gammatolerans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1362
-
D-glutamine at pH 8.5 and 85°C Thermococcus gammatolerans
2048
-
L-glutamine at pH 8.5 and 85°C Thermococcus gammatolerans
3378
-
D-asparagine at pH 8.5 and 85°C Thermococcus gammatolerans
5721
-
L-asparagine at pH 8.5 and 85°C Thermococcus gammatolerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Thermococcus gammatolerans

pH Range

pH Minimum pH Maximum Comment Organism
8 10 the enzyme shows more than 85% of relative activity at the range from pH 8.0-10.0 Thermococcus gammatolerans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17.6
-
D-glutamine at pH 8.5 and 85°C Thermococcus gammatolerans
51.9
-
L-glutamine at pH 8.5 and 85°C Thermococcus gammatolerans
140.2
-
D-asparagine at pH 8.5 and 85°C Thermococcus gammatolerans
572.1
-
L-asparagine at pH 8.5 and 85°C Thermococcus gammatolerans