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Literature summary for 3.5.1.1 extracted from
- Cloning, expression, purification and characterisation of Erwinia carotovora L-asparaginase in Escherichia coli (2014), Adv. Biomed. Res., 3, 82.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pectobacterium carotovorum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Pectobacterium carotovorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | Pectobacterium carotovorum | - |
L-aspartate + NH3 | - |
? |  |
| L-asparagine + H2O | Pectobacterium carotovorum NCYC 1526 | - |
L-aspartate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pectobacterium carotovorum | - |
- |
- |
| Pectobacterium carotovorum NCYC 1526 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni Sepharose column chromatography | Pectobacterium carotovorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Pectobacterium carotovorum NCYC 1526 | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Pectobacterium carotovorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ErA | - |
Pectobacterium carotovorum |
| L-asparaginase | - |
Pectobacterium carotovorum |
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Release 2023.1 (January 2023)
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