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Literature summary for 3.5.1.1 extracted from

  • Kavitha, A.; Vijayalakshmi, M.
    A study on L-asparaginase of Nocardia levis MK-VL_113 (2012), ScientificWorldJournal, 2012, 160434.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
additional information cell disrupting agents EDTA, lysozyme, and penicillin-G enhance the release of L-asparaginase from the cell culture Nocardia levis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Nocardia levis
-
 L-aspartate + NH3
-
 ?
L-asparagine + H2O Nocardia levis MK-VL_113
-
 L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Nocardia levis
-
 isolated from laterite soils of Guntur region, India
-
Nocardia levis MK-VL_113
-
 isolated from laterite soils of Guntur region, India
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture optimization of production of L-asparaginase by strain MK-VL_113. Optimal production at pH 7.0 and 30°C in a 72 h old culture in modified ISP-5 medium containing yeast extract, tryptone, or L-asparagine at 1-1.5% w/v as nitrogen source and 2% glycerol as carbon source, overview Nocardia levis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Nocardia levis L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Nocardia levis MK-VL_113 L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
L-asparaginase
-
 Nocardia levis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nocardia levis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Nocardia levis