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Literature summary for 3.5.1.1 extracted from

  • Warangkar, S.C.; Khobragade, C.N.
    Purification, characterization, and effect of thiol compounds on activity of the Erwinia carotovora L-asparaginase (2010), Enzyme Res., 2010, 165878.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates 112% at 1 mM Pectobacterium carotovorum
EDTA activates 117% at 1 mM Pectobacterium carotovorum
GSH causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation, activates 136% at 1 mM Pectobacterium carotovorum
L-cysteine activates 148% at 1 mM Pectobacterium carotovorum
L-cystine causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation Pectobacterium carotovorum
L-histidine activates 148% at 1 mM Pectobacterium carotovorum
L-methionine causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation Pectobacterium carotovorum
N-acetyl-L-cysteine causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation Pectobacterium carotovorum
SDS 78% inhibition at 3.0 mM, 119% activation at 2.0 mM Pectobacterium carotovorum

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inhibition at 0.5 mM Pectobacterium carotovorum
Ca2+ 22% inhibition at 1.0 mM Pectobacterium carotovorum
Cd2+ complete inhibition at 1.0 mM Pectobacterium carotovorum
Cu2+ complete inhibition at 1.0 mM Pectobacterium carotovorum
dithiothreitol 14% inhibition at 0.5 mM Pectobacterium carotovorum
Fe3+ 53% inhibition at 1.0 mM Pectobacterium carotovorum
H2O2 76% inhibition at 1.0 mM Pectobacterium carotovorum
Hg2+ complete inhibition at 1.0 mM Pectobacterium carotovorum
iodoacetamide complete inhibition at 0.5 mM Pectobacterium carotovorum
Mg2+ 76% inhibition at 1.0 mM Pectobacterium carotovorum
Mn2+ 29% inhibition at 1.0 mM Pectobacterium carotovorum
Ni2+ 49% inhibition at 1.0 mM Pectobacterium carotovorum
SDS 78% inhibition at 3.0 mM, 119% activation at 1.0 mM Pectobacterium carotovorum
Zn2+ 94% inhibition at 1.0 mM Pectobacterium carotovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Lineweaver-Burk and Michaelis-Menten kinetic analysis of activity, overview. Thiol compounds reduce the Km and increase the Vmax vlaues Pectobacterium carotovorum
0.098
-
L-asparagine pH 8.5, 37°C Pectobacterium carotovorum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates 133% at 1 mM Pectobacterium carotovorum
Na+ activates 117% at 1 mM Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Pectobacterium carotovorum
150000
-
gel filtration Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Pectobacterium carotovorum
-
L-aspartate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
isolated from soil after screening
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 752.9fold by ammonium sulfate fractionation, gel filtration, cation and anion exchange chromatography Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1034
-
purified enzyme, pH 8.5, 37°C Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
Pectobacterium carotovorum L-aspartate + NH3
-
?
L-asparagine + H2O specific for Pectobacterium carotovorum L-aspartate + NH3
-
?
additional information the hydrolysis efficiency of asparagine is at least 11925fold higher than that of L-glutamine Pectobacterium carotovorum ?
-
?

Subunits

Subunits Comment Organism
oligomer x * 37000, SDS-PAGE Pectobacterium carotovorum

Synonyms

Synonyms Comment Organism
L-asparaginase
-
Pectobacterium carotovorum
L-asparagine amino hydrolase
-
Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Pectobacterium carotovorum