Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates 112% at 1 mM | Pectobacterium carotovorum | |
EDTA | activates 117% at 1 mM | Pectobacterium carotovorum | |
GSH | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation, activates 136% at 1 mM | Pectobacterium carotovorum | |
L-cysteine | activates 148% at 1 mM | Pectobacterium carotovorum | |
L-cystine | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation | Pectobacterium carotovorum | |
L-histidine | activates 148% at 1 mM | Pectobacterium carotovorum | |
L-methionine | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation | Pectobacterium carotovorum | |
N-acetyl-L-cysteine | causes an increase in Vmax value and a decrease in Km value, nonessential mode of activation | Pectobacterium carotovorum | |
SDS | 78% inhibition at 3.0 mM, 119% activation at 2.0 mM | Pectobacterium carotovorum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | complete inhibition at 0.5 mM | Pectobacterium carotovorum | |
Ca2+ | 22% inhibition at 1.0 mM | Pectobacterium carotovorum | |
Cd2+ | complete inhibition at 1.0 mM | Pectobacterium carotovorum | |
Cu2+ | complete inhibition at 1.0 mM | Pectobacterium carotovorum | |
dithiothreitol | 14% inhibition at 0.5 mM | Pectobacterium carotovorum | |
Fe3+ | 53% inhibition at 1.0 mM | Pectobacterium carotovorum | |
H2O2 | 76% inhibition at 1.0 mM | Pectobacterium carotovorum | |
Hg2+ | complete inhibition at 1.0 mM | Pectobacterium carotovorum | |
iodoacetamide | complete inhibition at 0.5 mM | Pectobacterium carotovorum | |
Mg2+ | 76% inhibition at 1.0 mM | Pectobacterium carotovorum | |
Mn2+ | 29% inhibition at 1.0 mM | Pectobacterium carotovorum | |
Ni2+ | 49% inhibition at 1.0 mM | Pectobacterium carotovorum | |
SDS | 78% inhibition at 3.0 mM, 119% activation at 1.0 mM | Pectobacterium carotovorum | |
Zn2+ | 94% inhibition at 1.0 mM | Pectobacterium carotovorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk and Michaelis-Menten kinetic analysis of activity, overview. Thiol compounds reduce the Km and increase the Vmax vlaues | Pectobacterium carotovorum | |
0.098 | - |
L-asparagine | pH 8.5, 37°C | Pectobacterium carotovorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates 133% at 1 mM | Pectobacterium carotovorum | |
Na+ | activates 117% at 1 mM | Pectobacterium carotovorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Pectobacterium carotovorum |
150000 | - |
gel filtration | Pectobacterium carotovorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Pectobacterium carotovorum | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pectobacterium carotovorum | - |
isolated from soil after screening | - |
Purification (Comment) | Organism |
---|---|
native enzyme 752.9fold by ammonium sulfate fractionation, gel filtration, cation and anion exchange chromatography | Pectobacterium carotovorum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1034 | - |
purified enzyme, pH 8.5, 37°C | Pectobacterium carotovorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | specific for | Pectobacterium carotovorum | L-aspartate + NH3 | - |
? | |
additional information | the hydrolysis efficiency of asparagine is at least 11925fold higher than that of L-glutamine | Pectobacterium carotovorum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 37000, SDS-PAGE | Pectobacterium carotovorum |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase | - |
Pectobacterium carotovorum |
L-asparagine amino hydrolase | - |
Pectobacterium carotovorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pectobacterium carotovorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Pectobacterium carotovorum |