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Literature summary for 3.5.1.1 extracted from
- Recombinant expression and characterization of L-asparaginase II from a moderately thermotolerant bacterial isolate (2012), Appl. Biochem. Biotechnol., 167, 973-980.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ansB, cloning in Escherichia coli strain DH5alpha and expression as His6-tagged protein in Escherichia coli strain BL21(DE3) | unidentified proteobacterium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.89 | - |
L-asparagine | pH 7.0, 37°C, recombinant enzyme | unidentified proteobacterium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, recombinant His6-tagged enzyme, SDS-PAGE | unidentified proteobacterium |
| 150000 | - |
about, native PAGE | unidentified proteobacterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | unidentified proteobacterium | - |
L-aspartate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| unidentified proteobacterium | - |
Enterobacteriaceae, bacterium isolated from cow dung, gene ansB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | unidentified proteobacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
unidentified proteobacterium | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 36000, recombinant His6-tagged enzyme, SDS-PAGE | unidentified proteobacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-asparaginase II | - |
unidentified proteobacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
unidentified proteobacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range, sharp decrease in activity above 37°C | unidentified proteobacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the purified recombinant enzyme retains 38% of its initial activity after 10 min, 33% after 30 and 45 min, and 28% after 60 min of incubation | unidentified proteobacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
unidentified proteobacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | activity range, profile overview | unidentified proteobacterium |
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