Cloned (Comment) | Organism |
---|---|
expression of the mutant chimeric enzyme in the periplasm of strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric enzyme composed of asparaginase, a tetanus toxin peptide spacer, fragment 831-854, and the foreign cholesteryl ester transfer protein C-terminal fragment, targeting to and expression in the periplasm of Escherichia coli | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Escherichia coli | - |
L-aspartate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native wild-type and recombinant mutant chimeric enzyme from the periplasm of strain BL21(DE3) by periplasm preparation through osmotic shock, and anion exchange chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | potential rapid pepscan technique for antigen epitope mapping | Escherichia coli |
tetramer | homotetramer | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |