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Literature summary for 3.5.1.1 extracted from

  • Kotzia, G.A.; Labrou, N.E.
    Cloning, expression and characterisation of Erwinia carotovora L-asparaginase (2005), J. Biotechnol., 119, 309-323.
    View publication on PubMed
Search for more literature for 3.5.1.1

Application

Application Comment Organism
medicine the enzyme is used as therapeutic agent in the treatment of acute childhood lymphoblastic leukemia Pectobacterium carotovorum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pectobacterium carotovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamic analysis, kinetic study and molecular modelling Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Pectobacterium carotovorum
-
 L-aspartate + NH3
-
 ?
L-asparagine + H2O Pectobacterium carotovorum NCYC 1526
-
 L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
-
-
Pectobacterium carotovorum NCYC 1526
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity by cation exchange and L-asparagine affinity chromatography Pectobacterium carotovorum

Reaction

Reaction Comment Organism Reaction ID
L-asparagine + H2O = L-aspartate + NH3 kinetic model, substrate specificity, and catalytic mechanism, active site residues are Asp115 and Glu82, the structural transition of the enzyme is rate-limiting in the reaction, entropy changes are the main determinants for the substrate specificity Pectobacterium carotovorum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartate + NH3
-
 ?
L-asparagine + H2O
-
 Pectobacterium carotovorum NCYC 1526 L-aspartate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
ErA
-
 Pectobacterium carotovorum
L-asparaginase
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
-
 Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Pectobacterium carotovorum