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Literature summary for 3.5.1.1 extracted from
- Immobilization of L-asparaginase on the microparticles of the natural silk sericin protein and its characters (2004), Biomaterials, 25, 3751-3759.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | L-asparaginase is an anti-leukemic enzyme | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | covalent immobilization of L-asparaginase on poorly soluble microparticles of the natural silk sericin protein, MW 50-200 kDa, from Bomby mori, best at 0.15% glutaraldehyde in 50 mM citrate buffer, pH 8.6, method optimization and biochemical properties of the enzyme-conjugate, overview | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| half-life of immobilized enzyme at 37°C, pH 8.6 is 8 days | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Glutaraldehyde | inhibits the enzyme during immobilization at concentrations above 0.2% | Escherichia coli |  |
| Trypsin | the free enzyme is completely inactivated by trypsin within 10 min, while the immobilized enzyme is stable for 240 min with loss of 30% activity | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the Km of the immobilized enzyme is 8fold lower compared to the free enzyme | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 138000 | 141000 | - |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | Escherichia coli | - |
L-aspartate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Escherichia coli | L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | homotetramer with one active center | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-ASNase | - |
Escherichia coli |
| L-asparaginase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
free enzyme | Escherichia coli |
| 60 | - |
immobilized enzyme | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | immobilized enzyme, 60% of maximal activity at 70°C | Escherichia coli |
| 30 | 60 | free enzyme, inactivation at 70°C | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, about 20% reduced activity, immobilized enzyme and free enzyme | Escherichia coli |
| 60 | - |
30 min, about 70% reduced activity, immobilized enzyme and free enzyme | Escherichia coli |
| 70 | - |
30 min, about 80% reduced activity, immobilized enzyme and free enzyme | Escherichia coli |
| 80 | - |
30 min, inactivation of immobilized enzyme and free enzyme | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at, immobilized and free enzyme | Escherichia coli |
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