Activating Compound | Comment | Organism | Structure |
---|---|---|---|
albumin | 0.1 mg/ml, 3fold activation of L-asparaginase II | Tetrahymena thermophila | |
sorbitol | 30% w/v, activates more than 10fold | Tetrahymena thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phospholipase C | - |
Tetrahymena thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
L-Asn | asparaginase I and II | Tetrahymena thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
rough endoplasmic reticulum | - |
Tetrahymena thermophila | 5791 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 40-45% activation of L-asparaginase I and II | Tetrahymena thermophila | |
Mg2+ | 5-10% activation | Tetrahymena thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, L-asparaginase I, SDS-PAGE | Tetrahymena thermophila |
93000 | - |
x * 93000, L-asparaginase II, SDS-PAGE | Tetrahymena thermophila |
200000 | - |
L-asparaginase II, gel filtration | Tetrahymena thermophila |
1000000 | - |
L-asparaginase I, gel filtration | Tetrahymena thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetrahymena thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
L-asparaginase I and II | Tetrahymena thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.1 | - |
- |
Tetrahymena thermophila |
59.6 | - |
- |
Tetrahymena thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asn + H2O | - |
Tetrahymena thermophila | L-Asp + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 93000, L-asparaginase II, SDS-PAGE | Tetrahymena thermophila |
? | x * 50000, L-asparaginase I, SDS-PAGE | Tetrahymena thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
L-asparaginase I and II | Tetrahymena thermophila |