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Literature summary for 3.5.1.1 extracted from

  • Tsavdaridis, I.K.; Triantafillidou, D.C.; Kyriakidis, D.A.
    Two forms of L-asparaginase in Tetrahymena thermophila (1994), Biochem. Mol. Biol. Int., 32, 67-77.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
albumin 0.1 mg/ml, 3fold activation of L-asparaginase II Tetrahymena thermophila
sorbitol 30% w/v, activates more than 10fold Tetrahymena thermophila

Inhibitors

Inhibitors Comment Organism Structure
Phospholipase C
-
Tetrahymena thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1
-
L-Asn asparaginase I and II Tetrahymena thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
rough endoplasmic reticulum
-
Tetrahymena thermophila 5791
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 40-45% activation of L-asparaginase I and II Tetrahymena thermophila
Mg2+ 5-10% activation Tetrahymena thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, L-asparaginase I, SDS-PAGE Tetrahymena thermophila
93000
-
x * 93000, L-asparaginase II, SDS-PAGE Tetrahymena thermophila
200000
-
L-asparaginase II, gel filtration Tetrahymena thermophila
1000000
-
L-asparaginase I, gel filtration Tetrahymena thermophila

Organism

Organism UniProt Comment Textmining
Tetrahymena thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
L-asparaginase I and II Tetrahymena thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.1
-
-
Tetrahymena thermophila
59.6
-
-
Tetrahymena thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asn + H2O
-
Tetrahymena thermophila L-Asp + NH3
-
?

Subunits

Subunits Comment Organism
? x * 93000, L-asparaginase II, SDS-PAGE Tetrahymena thermophila
? x * 50000, L-asparaginase I, SDS-PAGE Tetrahymena thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
L-asparaginase I and II Tetrahymena thermophila