Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.1 extracted from

  • Raha, S.K.; Roy, S.K.; Dey, S.K.; Chakrabarty, S.L.
    Purification and properties of an L-asparaginase from Cylindrocarpon obtusisporum MB-10 (1990), Biochem. Int., 21, 987-1000.
    View publication on PubMed
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin slightly enhances activity Neonectria obtusispora
EDTA enhances activity Neonectria obtusispora
GSH enhances activity Neonectria obtusispora
L-Cys enhances activity Neonectria obtusispora
L-His enhances activity Neonectria obtusispora

General Stability

General Stability Organism
stable to repeated freezing and thawing Neonectria obtusispora

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Neonectria obtusispora
D-Asn
-
 Neonectria obtusispora
Fe2+
-
 Neonectria obtusispora
Hg2+
-
 Neonectria obtusispora
iodoacetamide
-
 Neonectria obtusispora
Ni2+
-
 Neonectria obtusispora
PCMB
-
 Neonectria obtusispora
SDS
-
 Neonectria obtusispora
Triton X-100
-
 Neonectria obtusispora
Urea
-
 Neonectria obtusispora
Zn2+ slight inhibition Neonectria obtusispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 L-asparagine
-
 Neonectria obtusispora

Metals/Ions

Metals/Ions Comment Organism Structure
CN- enhances activity Neonectria obtusispora
I- enhances activity Neonectria obtusispora
additional information enzyme is not a metalloprotein Neonectria obtusispora
PO43- enhances activity Neonectria obtusispora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
216000
-
 gel filtration Neonectria obtusispora
543000
-
 4 * 543000, SDS-PAGE Neonectria obtusispora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Asn + H2O Neonectria obtusispora constitutive enzyme L-Asp + NH3
-
 ?
L-Asn + H2O Neonectria obtusispora MB-20 constitutive enzyme L-Asp + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Neonectria obtusispora
-
 MB-20
-
Neonectria obtusispora MB-20
-
 MB-20
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 37.3% w/w carbohydrate Neonectria obtusispora

Purification (Commentary)

Purification (Comment) Organism
-
 Neonectria obtusispora

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Neonectria obtusispora

Storage Stability

Storage Stability Organism
-20°C, stable Neonectria obtusispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Asn + H2O no activity Neonectria obtusispora D-Asp + NH3
-
 ?
D-Asn + H2O no activity Neonectria obtusispora MB-20 D-Asp + NH3
-
 ?
L-Asn + H2O
-
 Neonectria obtusispora L-Asp + NH3
-
 ?
L-Asn + H2O constitutive enzyme Neonectria obtusispora L-Asp + NH3
-
 ?
L-Asn + H2O
-
 Neonectria obtusispora MB-20 L-Asp + NH3
-
 ?
L-Asn + H2O constitutive enzyme Neonectria obtusispora MB-20 L-Asp + NH3
-
 ?
L-Gln + H2O no activity Neonectria obtusispora L-Glu + NH3
-
 ?
L-Gln + H2O no activity Neonectria obtusispora MB-20 L-Glu + NH3
-
 ?
poly-L-asparagine + H2O at 21.7% of the activity with L-Asn Neonectria obtusispora ?
-
 ?
poly-L-asparagine + H2O at 21.7% of the activity with L-Asn Neonectria obtusispora MB-20 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 543000, SDS-PAGE Neonectria obtusispora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Neonectria obtusispora

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 42 20°C: about 30% of maximal activity, 42°C: about 35% of maximal activity Neonectria obtusispora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 30 min, stable Neonectria obtusispora
40
-
 30 min, 20% loss of activity Neonectria obtusispora
50
-
 30 min, complete loss of activity Neonectria obtusispora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Neonectria obtusispora