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Literature summary for 3.4.99.B1 extracted from

  • Kyro, K.; Manandhar, S.P.; Mullen, D.; Schmidt, W.K.; Distefano, M.D.
    Photoaffinity labeling of Ras converting enzyme 1 (Rce1p) using a benzophenone-containing peptide substrate (2010), Bioorg. Med. Chem., 18, 5675-5684.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.99.B1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Saccharomyces cerevisiae
N6-[1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl]-N2-{4,15-dioxo-19-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-8,11,14-trioxa-5-azanonadecan-1-oyl}-L-lysyl-L-lysyl-L-seryl-L-lysyl-L-threonyl-L-lysyl-S-{(2E)-4-[(3-benzoylbenzyl)oxy]-3-methylbut-2-en-1-yl}-L-cysteinyl-N6-{3-[hydroxy(oxo)ammonio]-5-nitrophenyl}-L-lysyl-L-isoleucyl-L-methionine labels the active site by crosslinking to the biotinylated probe, competitive to Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]-L-Cys-N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met Saccharomyces cerevisiae
Zn2+ at high concentration Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E)-4-[(3-benzoylbenzyl)oxy]-3-methylbut-2-en-1-yl]-L-Cys-N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met pH 7.5, 30°C Saccharomyces cerevisiae
0.0064
-
 Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]-L-Cys-N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met pH 7.5, 30°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Saccharomyces cerevisiae 16020
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 wild-type strain SM3614
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E)-4-[(3-benzoylbenzyl)oxy]-3-methylbut-2-en-1-yl]-L-Cys-N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met + H2O i.e. 2-aminobenzoyl-KSKTKC(3-methylbenzophenone prenyl ether)K(dinitrophenyl)IM, an aryl ketone-containing peptide photophore, incorporating a benzophenone-modified isoprenoid, synthesis and structure, mass spectrometrical analysis, overview Saccharomyces cerevisiae Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E)-4-[(3-benzoylbenzyl)oxy]-3-methylbut-2-en-1-yl]-L-Cys + N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met mass spectrometrical product analysis ?
Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]-L-Cys-N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met + H2O the isoprenylated peptide substrate is derived from the C-terminal sequence of mammalian K-Ras4B, synthesized from 2-aminobenzoyl-KSKTKCK(dinitrophenyl)IM, synthesis and structure, mass spectrometrical analysis, overview Saccharomyces cerevisiae Abz-L-Lys-L-Ser-L-Lys-L-The-L-Lys-S-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]-L-Cys + N6-(3,5-dinitrophenyl)-L-LysL-L-Ile-L-Met mass spectrometrical product analysis ?
additional information Rce1p proteolysis requires histidine and glutamate residues, sequential analysis of Rce1p identifies invariant residues reminiscent of a consensus HEXXH motif found in metalloproteases Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Ras converting enzyme 1
-
 Saccharomyces cerevisiae
Rce1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae