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Literature summary for 3.4.25.2 extracted from
- Kinetics of protein substrate degradation by HslUV (2004), J. Struct. Biol., 146, 141-147.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Haemophilus influenzae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Haemophilus influenzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
SulA mutant P8L | pH 5.5, 37°C, recombinant enzyme | Haemophilus influenzae | |
| 0.0023 | - |
SulA | pH 5.5, 37°C, wild-type SulA, recombinant enzyme | Haemophilus influenzae | |
| 0.0032 | - |
SulA mutant I37V | pH 5.5, 37°C, recombinant enzyme | Haemophilus influenzae | |
| 0.0034 | - |
SulA mutant F10A | pH 5.5, 37°C, recombinant enzyme | Haemophilus influenzae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Haemophilus influenzae |  |
| Mg2+ | - |
Haemophilus influenzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| SulA + H2O | Haemophilus influenzae | specific substrate degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Haemophilus influenzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme degrades only the SulA moiety of recombinant fusion proteins, the fused proteins, e.g. the green fluorescent protein, are not hydrolyzed | Haemophilus influenzae | ? | - |
? | |
| SulA + H2O | specific substrate degradation | Haemophilus influenzae | ? | - |
? | |
| SulA + H2O | specific substrate degradation, activities with SulA mutant protein substrates F10A, I37V, and P8L | Haemophilus influenzae | ? | - |
? | |
| SulA mutant F10A + H2O | - |
Haemophilus influenzae | ? | - |
? | |
| SulA mutant I37V + H2O | - |
Haemophilus influenzae | ? | - |
? | |
| SulA mutant P8L + H2O | - |
Haemophilus influenzae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | the HslU12HslV12 protease-chaperone complex | Haemophilus influenzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HslUV | - |
Haemophilus influenzae |
| HslUV protease-chaperone complex | - |
Haemophilus influenzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Haemophilus influenzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
SulA mutant P8L | pH 5.5, 37°C, recombinant enzyme | Haemophilus influenzae | |
| 0.05 | - |
SulA | pH 5.5, 37°C, wild-type SulA, recombinant enzyme | Haemophilus influenzae | |
| 0.063 | - |
SulA mutant F10A | pH 5.5, 37°C, recombinant enzyme | Haemophilus influenzae | |
| 0.068 | - |
SulA mutant I37V | pH 5.5, 37°C, recombinant enzyme | Haemophilus influenzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Haemophilus influenzae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Haemophilus influenzae | |
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