Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.25.1 extracted from

  • Book, A.J.; Gladman, N.P.; Lee, S.S.; Scalf, M.; Smith, L.M.; Vierstra, R.D.
    Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes (2010), J. Biol. Chem., 285, 25554-25569.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.25.1

Cloned(Commentary)

Cloned (Comment) Organism
26S proteasome subunits phylogenetic analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information identification of pag1-1, pa200-1-6, and T (transfer)-DNA insertion mutants in the Arabidopsis thaliana ecotype Col-0 in a T-DNA library, expression of HA-, Myc-, or FLAG-tagged PAG1 derivatives, gene At2g27020 or UniProt ID O23715. The PAG1-FLAG protein successfully integrated into the 26 S proteasome complex without perturbing CP/RP assembly or the phenotype Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
MG132 CP inhibitor Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview ?
-
 ?
additional information Arabidopsis thaliana Col-0 several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana Col-0
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information a number of core and regulatory particle subunits undergo one or more posttranslational modifications, including partial proteolytic cleavage, acetylation, and ubiquitylation, mass spectrometric analysis of the complex, overview Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
development of an affinity method to rapidly purify intact epitope-tagged 26S proteasomes using mutant homozygous PAG1-FLAG pag1-1 line Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O trypsin-like activity Arabidopsis thaliana Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O trypsin-like activity Arabidopsis thaliana Col-0 Boc-Leu-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview Arabidopsis thaliana ?
-
 ?
additional information several other proteins associate with the Arabidopsis thaliana proteasome, including the PBAC2 assembly chaperonin, the associated DSS1/Sem1/RPN15 protein, the deubiquitylating enzyme UBP16, and the alternative activator PA200, genetic analysis of PA200 in Arabidopsis, overview Arabidopsis thaliana Col-0 ?
-
 ?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Arabidopsis thaliana succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Arabidopsis thaliana Col-0 succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
More the 26 S proteasome is composed of two subparticles, the 20S core protease that compartmentalizes the protease active sites and the 19S regulatory particle that recognizes and translocates appropriate substrates into the core protease lumen for breakdown. The 2.5-MDa core protease-regulatory particle complex is actually a heterogeneous set of particles assembled with paralogous pairs for most subunits, mass spectrometric analysis of the complex, overview Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
additional information activity and subunit composition of the 26 S proteasomes in plants, detailed overview. The 26 S proteasome is composed of two subparticles, the 20 S core protease that compartmentalizes the protease active sites and the 19 S regulatory particle that recognizes and translocates appropriate substrates into the core protease lumen for breakdown. A diverse and highly dynamic population of proteasomes is assembled in plants, which may expand the target specificity and functions of intracellular proteolysis, overview Arabidopsis thaliana