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Literature summary for 3.4.25.1 extracted from

  • Kloss, A.; Meiners, S.; Ludwig, A.; Dahlmann, B.
    Multiple cardiac proteasome subtypes differ in their susceptibility to proteasome inhibitors (2010), Cardiovasc. Res., 85, 367-375.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
bortezomib an N-terminally blocked Phe-Leu dipeptide inhibitor containing a C-terminal boronic acid, inhibits chymotrypsin-likeand caspase-like actitivities of the proteasome, inhibitory pattern with the chymotrypsin-like activity of the intermediate-proteasome subtypes, overview Rattus norvegicus
MG132 i.e. Z-Leu-Leu-Leu-aldehyde, inhibits the chymotrypsin-like proteasome activity, inhibitory pattern with the chymotrypsin-like activity of the intermediate-proteasome subtypes, overview. Does not affect the caspase-like activity of subtypes II-2, I,-3, and I-4 at all Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
native 20S proteasomes from hearts by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
heart specific activities of the three standard proteasome subtypes II-2, II-3, and II-4 from rat heart exhibit high chymotrypsin-like activity, whereas the trypsin-like activity of these three subtypes account for only 1-2% compared with the chymotrypsin-like activity, their caspase-like activities overexceed the latter by 35-50%, overview Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-VGR-4-methyl-7-amido-coumarin + H2O trypsin-like proteasome activity Rattus norvegicus benzoyl-VGR + 4-methyl-7-amino-coumarin
-
?
N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O chymotrypsin-like proteasome activity Rattus norvegicus N-succinyl-LLVY + 7-amino-4-methylcoumarin
-
?
Z-LLE-4-methyl-7-amido-coumarin + H2O post-acidic proteasome activity Rattus norvegicus Z-LLE + 4-methyl-7-amino-coumarin
-
?

Synonyms

Synonyms Comment Organism
20S proteasome
-
Rattus norvegicus
proteasome
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus