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Literature summary for 3.4.25.1 extracted from

  • Castro-Borges, W.; Cartwright, J.; Ashton, P.D.; Braschi, S.; Guerra Sa, R.; Rodrigues, V.; Wilson, R.A.; Curwen, R.S.
    The 20S proteasome of Schistosoma mansoni: a proteomic analysis (2007), Proteomics, 7, 1065-1075.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
SDS increased activity in the presence of 0.02% SDS Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, CHTII hydroxyapatite column chromatography, and phenyl-Sepharose column chromatograpyh Schistosoma mansoni

Subunits

Subunits Comment Organism
More on 1-D SDS-PAGE five to seven bands of molecular weight range 20 to 30 kDa that prove to be entirely proteasome subunits, when the constituents are separated by 2-D SDS-PAGE, an unexpectedly large number of subunits is observed with at least 46 spots present versus an absolute minimum of 14 possible for a 20S core Schistosoma mansoni

Synonyms

Synonyms Comment Organism
20S proteasome
-
Schistosoma mansoni

pI Value

Organism Comment pI Value Maximum pI Value
Schistosoma mansoni subunits show pI-values from 4.5 to 7.0 by isoelectric focusing 7 4.5