Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Renatured (Comment) | Organism |
---|---|
denaturation of the proteasome by 4 M urea followed by high-temperature dialysis. The wild-type temperature optimum is restored, but only if proteasome subunits are denatured and refolded prior to assembly | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
20S proteasome | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
recombinant enzyme | Methanocaldococcus jannaschii |
119 | - |
wild-type enzyme | Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 100 | 80°C: about 60% of maximal activity, 100°C: about 45% of maximal activity, recombinant enzyme, hydrolysis of benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide | Methanocaldococcus jannaschii |
100 | 130 | 100°C: about 40% of maximal activity, 130°C: about 20% of maximal activity, wild-type enzyme, hydrolysis of benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide | Methanocaldococcus jannaschii |