Literature summary for 3.4.25.1 extracted from

Thomas, A.R.; Oosthuizen, V.; Naude, R.J.; Muramoto, K.
Purification and characterization of the 20S proteasome from ostrich skeletal muscle (2002), Biol. Chem., 383, 1267-1270.

Search for more literature for 3.4.25.1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.051	-	benzyloxycarbonyl-RR-7-amido-4-methylcoumarin	-	Struthio camelus
0.248	-	benzyloxycarbonyl-ARR-7-amido-4-methylcoumarin	-	Struthio camelus
0.255	-	benzyloxycarbonyl-LLE-2-naphthylamide	-	Struthio camelus
0.263	-	succinyl-LLVY-7-amido-4-methylcoumarin	-	Struthio camelus
0.751	-	benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin	-	Struthio camelus
0.798	-	benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin	-	Struthio camelus
1.504	-	succinyl-AAF-7-amido-4-methylcoumarin	-	Struthio camelus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
700000	-	-	Struthio camelus

Organism

Organism	UniProt	Comment	Textmining
Struthio camelus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Struthio camelus

Source Tissue

Source Tissue	Comment	Organism	Textmining
skeletal muscle	-	Struthio camelus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-ARR-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
benzyloxycarbonyl-FR-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
benzyloxycarbonyl-Gly-Gly-Leu-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
benzyloxycarbonyl-LLE-2-naphthylamide + H2O	-	Struthio camelus	?	-	?
benzyloxycarbonyl-RR-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
H-VLK-7-amido-4-methylcoumarin + H2O	very-low activity	Struthio camelus	?	-	?
N-succinyl-LLVY-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
succinyl-AAF-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?
succinyl-LLVY-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	succinyl-LLVY + 7-amino-4-methylcoumarin	-	?
tert-butyloxycarbonyl-LRR-7-amido-4-methylcoumarin + H2O	-	Struthio camelus	?	-	?

Subunits

Subunits	Comment	Organism
More	a ladder of 22200-33500 Da bands detected on SDS-PAGE	Struthio camelus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	tryptic-like activity	Struthio camelus
60	-	chymotryptic-like activity	Struthio camelus
70	-	peptidylglutamyl peptide hydrolase activity	Struthio camelus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	stable up to, trypsin-like activity	Struthio camelus
60	-	stable up to, chymotrypsin-like activity and peptidylglutamyl peptide hydrolase activity	Struthio camelus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	succinyl-AAF-7-amido-4-methylcoumarin	-	Struthio camelus
0.052	2.1	benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin	-	Struthio camelus
0.143	-	benzyloxycarbonyl-RR-7-amido-4-methylcoumarin	-	Struthio camelus
0.332	-	benzyloxycarbonyl-LLE-2-naphthylamide	-	Struthio camelus
2.94	-	benzyloxycarbonyl-ARR-7-amido-4-methylcoumarin	-	Struthio camelus
6.08	-	succinyl-LLVY-7-amido-4-methylcoumarin	-	Struthio camelus
16.9	-	benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin	-	Struthio camelus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	peptidylglutamyl peptide hydrolase activity	Struthio camelus
9	-	chymotryptic-like activity	Struthio camelus
11	-	tryptic-like activity	Struthio camelus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Struthio camelus	-	-	6.67

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Release 2023.1 (January 2023)