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Literature summary for 3.4.25.1 extracted from

  • Maupin-Furlow, J.A.; Aldrich, H.C.; Ferry, J.G.
    Biochemical characterization of the 20S proteasome from the methanoarchaeon Methanosarcina thermophila (1998), J. Bacteriol., 180, 1480-1487.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.25.1

Activating Compound

Activating Compound Comment Organism Structure
SDS stimulates chymotrypsin-like activity Methanosarcina thermophila

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
Cl- inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
K+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
Mg2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
Mn2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
SDS inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
Zn2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates chymotrypsin-like activity Methanosarcina thermophila
Cl- stimulates chymotrypsin-like activity Methanosarcina thermophila
K+ stimulates chymotrypsin-like activity Methanosarcina thermophila
Mg2+ stimulates chymotrypsin-like activity Methanosarcina thermophila
Mn2+ stimulates chymotrypsin-like activity Methanosarcina thermophila
Zn2+ stimulates chymotrypsin-like activity Methanosarcina thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 alpha, beta, 14 * 24000 + 14 * 22000, SDS-PAGE, alpha7,beta7,beta7,alpha7 in the 20S proteasome Methanosarcina thermophila
24000
-
 alpha, beta, 14 * 24000 + 14 * 22000, SDS-PAGE, alpha7,beta7,beta7,alpha7 in the 20S proteasome Methanosarcina thermophila
654000
-
 gel filtration Methanosarcina thermophila

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Reaction

Reaction Comment Organism Reaction ID
cleavage of peptide bonds with very broad specificity chymotrypsin-like activity and peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0013
-
 substrate succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin Methanosarcina thermophila
0.0091
-
 substrate carbobenzoxy-Leu-Leu-Glu-beta-naphthylamide Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O
-
 Methanosarcina thermophila ?
-
 ?
Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
t-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?

Subunits

Subunits Comment Organism
polymer alpha, beta, 14 * 24000 + 14 * 22000, SDS-PAGE, alpha7,beta7,beta7,alpha7 in the 20S proteasome Methanosarcina thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 chymotrypsin-like activity Methanosarcina thermophila