Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Protein kinase C | phosphorylation of the enzyme by protein kinase C activates the enzyme, phosphorylation is stimualted by phorbol 12-myristate 13-acetate | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of soluble enzyme form, nucleotides 1-1253, with a Myc-epitope in COS-7 cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-directed mutagenesis of the furin cleavage site and of the cysteine-switch region results in highly reduced precursor processing, construction of a deletion mutant resulting in a soluble enzyme form | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Mus musculus | |
batimastat | - |
Mus musculus | |
brefeldin A | blocks processing of the enzyme precursor by blocking the vesicle budding in the endoplasmic reticulum | Mus musculus | |
CGS 27023 | - |
Mus musculus | |
coban | complete blockage of enzyme processing at 0.025 mg/ml | Mus musculus | |
marimastat | - |
Mus musculus | |
PKVCGYLK | inhibition less tan 20% at 0.1 mM | Mus musculus | |
PLKCGVSN | inhibition less tan 20% at 0.1 mM | Mus musculus | |
PLRCGVSN | inhibition less tan 20% at 0.1 mM | Mus musculus | |
QGGCADHS | inhibition less tan 20% at 0.1 mM | Mus musculus | |
QGLCGSQH | inhibition less tan 20% at 0.1 mM | Mus musculus | |
TNF-alpha protease inhibitor | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | anchored on the cell surface | Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | metalloprotease | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE | Mus musculus |
84000 | - |
x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | regulation of metalloprotease desintegrins via a cysteine-switch mechanism | ? | - |
? | |
proteins + H2O | Mus musculus | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation of endopeptidase H-sensitive precursor and mature enzyme | Mus musculus |
proteolytic modification | cleavage of pro-domain by furin-type pro-protein convertase before the protein is secreted to the cell surface | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from COS-7 cells | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
EVHHQKLVFFAE + H2O | peptides derived from beta-amyloid precursor protein, comprises the predicted cleavage site | Mus musculus | EVHH + QKLVFFAE | - |
? | |
insulin B chain + H2O | cleavage sites are Tyr16-Leu17 and Tyr26-Thr27 | Mus musculus | 3 peptides | - |
? | |
additional information | regulation of metalloprotease desintegrins via a cysteine-switch mechanism | Mus musculus | ? | - |
? | |
proteins + H2O | - |
Mus musculus | peptides | - |
? | |
SMAPGAVHLPQP + H2O | peptides derived from P75 TNF-receptor, comprises the predicted cleavage site | Mus musculus | SMAPGAVH + LPQP | - |
? | |
SPLAQAVRSSSR + H2O | peptides derived from TNF-alpha, comprises the predicted cleavage site | Mus musculus | SPLA + QAVRSSSR | - |
? | |
SPLAQAVRSSSR + H2O | peptides derived from TNF-alpha, comprises the predicted cleavage site | Mus musculus | SPLAQAVRS + SSR | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, processed enzyme, x * 12000, enzyme proform, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
M12.209 | Merops-ID | Mus musculus |
MDC9 | - |
Mus musculus |
metallo protease desintegrin 9 | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
batimastat | pH 7.5 | Mus musculus | |
0.000017 | - |
TNF-alpha protease inhibitor | pH 7.5 | Mus musculus | |
0.000054 | - |
CGS 27023 | pH 7.5 | Mus musculus | |
0.00027 | - |
marimastat | pH 7.5 | Mus musculus | |
0.004 | - |
PKVCGYLK | pH 7.5 | Mus musculus | |
0.01 | - |
PLRCGVSN | pH 7.5 | Mus musculus | |
0.037 | - |
PLKCGVSN | pH 7.5 | Mus musculus | |
0.15 | - |
QGLCGSQH | pH 7.5 | Mus musculus | |
0.15 | - |
QGGCADHS | pH 7.5 | Mus musculus |