Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B7 extracted from

  • Mu, T.; Liang, W.; Ju, Y.; Wang, Z.; Wang, Z.; Roycik, M.D.; Sang, Q.X.; Yu, D.; Xiang, H.; Fang, X.
    Efficient soluble expression of secreted matrix metalloproteinase 26 in Brevibacillus choshinensis (2013), Protein Expr. Purif., 91, 125-133.
    View publication on PubMed
Search for more literature for 3.4.24.B7

Application

Application Comment Organism
synthesis use of Brevibacillus choshinensis as the host system for the soluble and active expression of MMP26. The enzyme is secreted in soluble form in the supernatant of cell culture medium. The yields of purified proform of MMP26 and catalytic form of MMP-26 are 12 and 18 mg/L, respectively, with high purity and homogeneity. Both pro- and catalytic form show gelatin zymography activity and the purified catalytic form has high enzymatic activity against DQ-gelatin substrate. Expression using several widely used expression vectors in Escheriochia coli cells results in insoluble expressions or soluble expressions with little catalytic activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Brevibacillus choshinensis Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform Homo sapiens
28000
-
 x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NRE1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
133000000
-
 fluorescent units/mg, pH 7.5, 22°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DQ-gelatin + H2O fluorescein conjugated substrate from pig skin Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform Homo sapiens