Application | Comment | Organism |
---|---|---|
synthesis | use of Brevibacillus choshinensis as the host system for the soluble and active expression of MMP26. The enzyme is secreted in soluble form in the supernatant of cell culture medium. The yields of purified proform of MMP26 and catalytic form of MMP-26 are 12 and 18 mg/L, respectively, with high purity and homogeneity. Both pro- and catalytic form show gelatin zymography activity and the purified catalytic form has high enzymatic activity against DQ-gelatin substrate. Expression using several widely used expression vectors in Escheriochia coli cells results in insoluble expressions or soluble expressions with little catalytic activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Brevibacillus choshinensis | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform | Homo sapiens |
28000 | - |
x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NRE1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133000000 | - |
fluorescent units/mg, pH 7.5, 22°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DQ-gelatin + H2O | fluorescein conjugated substrate from pig skin | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, SDS-PAGE of catalytic form, x * 28000, SDS-PAGE of His-tagged proform | Homo sapiens |