Literature summary for 3.4.24.B7 extracted from

Lee, S.; Park, H.I.; Sang, Q.X.
Calcium regulates tertiary structure and enzymatic activity of human endometase/matrilysin-2 and its role in promoting human breast cancer cell invasion (2007), Biochem. J., 403, 31-42.

Search for more literature for 3.4.24.B7

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Human breast cancer cell line, MDA-MB-231, transfected with wild-type MMP-26 cDNA shows a calcium-dependent invasive potential when compared with controls that were transfected with an inactive form of MMP-26 (E209A)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D114A	mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis	Homo sapiens
D114A	mutation induces enhanced affinity for the Ca2+ ion or an irreversible loss of enzymatic activity triggered by low-affinity calcium binding respectively	Homo sapiens
D165A	very low rates of hydrolysis that are less than 5% of that seen for wild-type MMP-26	Homo sapiens
E191A	very low rates of hydrolysis that are less than 5% of that seen for wild-type MMP-26	Homo sapiens
E209A	inactive mutant enzyme	Homo sapiens
K189E	calcium-independent high invasiveness is observed in the K189E mutant MDA-MB-231 cell line	Homo sapiens
K189E	mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis	Homo sapiens
K189E	mutation induces enhanced affinity for the Ca2+ ion or an irreversible loss of enzymatic activity triggered by low-affinity calcium binding respectively	Homo sapiens
V184D	mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	MMP-26 has one high-affinity and one low-affinity calcium binding site. High-affinity calcium binding is restored at physiologically low calcium conditions with a calcium-dissociation constant of 63 nM without inducing secondary and tertiary structural changes. Low-affinity calcium binding is restored at relatively high calcium concentrations and shows a low-affinity calcium-dissociation constant value of 0.12 mM, which is accompanied with the recovery of enzymatic activity reversibly and tertiary structural changes, but without secondary structural rearrangements	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Synonyms

Synonyms	Comment	Organism
endometase/matrilysin-2	-	Homo sapiens
MMP-26	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	high-affinity calcium binding protects MMP-26 against thermal denaturation	Homo sapiens

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Release 2023.1 (January 2023)