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Literature summary for 3.4.24.B6 extracted from

  • Khan, F.; Liu, H.; Reyes, A.; Witkowska, H.E.; Martinez-Avila, O.; Zhu, L.; Li, W.; Habelitz, S.
    The proteolytic processing of amelogenin by enamel matrix metalloproteinase (MMP-20) is controlled by mineral ions (2013), Biochim. Biophys. Acta, 1830, 2600-2607.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0195
-
 amelogenin presence of 33.4 mM CaCl2 and 20.9 mM KH2PO4, pH 7.4, 37°C Homo sapiens
0.0299
-
 amelogenin pH 7.4, 37°C Homo sapiens
0.0407
-
 amelogenin presence of 3.34 mM CaCl2 and 2.09 mM KH2PO4, pH 7.4, 37°C Homo sapiens
0.0634
-
 amelogenin presence 20.9 mM KH2PO4, pH 7.4, 37°C Homo sapiens
0.585
-
 amelogenin presence of 33.4 mM CaCl2, pH 7.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60882
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amelogenin + H2O
-
 Homo sapiens amelogenin fragments rH163 + rH146 in high phosphates conditions (20.9 mM KH2PO4) no cleavage site is observed in the central region of residues Q54–P146, which also shows the slowest cleavage rate with the maximum accumulation of fragment rH163 at 4 h time-point. In the no calcium and phosphates conditions, the accumulation of rH163 reaches the maximum amount at 1 h and the cleavage in the central region Q54-P146 appears only after 5 h. Under high calcium conditions (33.4 mM CaCl2), the fastest cleavage rate is observed and also few exclusive and additional cleavage sites at T61, H67. Other conditions also show some exclusive cleavage sites such as P2 for no calcium and phosphates, P50 for low calcium and phosphates and G10 for high calcium and phosphates. The N-terminal positions Y11, N13, F14, S15, E17, K23, W24, P33, G42, and S53 and the C-terminal positions P155 and T158 are cleaved in all conditions used ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
 amelogenin presence of 33.4 mM CaCl2 and 20.9 mM KH2PO4, pH 7.4, 37°C Homo sapiens
0.056
-
 amelogenin presence 20.9 mM KH2PO4, pH 7.4, 37°C Homo sapiens
0.058
-
 amelogenin pH 7.4, 37°C Homo sapiens
0.068
-
 amelogenin presence of 3.34 mM CaCl2 and 2.09 mM KH2PO4, pH 7.4, 37°C Homo sapiens
0.77
-
 amelogenin presence of 33.4 mM CaCl2, pH 7.4, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.8
-
 amelogenin presence 20.9 mM KH2PO4, pH 7.4, 37°C Homo sapiens
1.3
-
 amelogenin presence of 33.4 mM CaCl2, pH 7.4, 37°C Homo sapiens
1.6
-
 amelogenin presence of 3.34 mM CaCl2 and 2.09 mM KH2PO4, pH 7.4, 37°C Homo sapiens
1.6
-
 amelogenin presence of 33.4 mM CaCl2 and 20.9 mM KH2PO4, pH 7.4, 37°C Homo sapiens
1.9
-
 amelogenin pH 7.4, 37°C Homo sapiens