Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue cells | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amelogenin + H2O | Sus scrofa | most of the secreted substrate is cleaved after Ser148 to generate the 20000 Da amelogenin (Met1-Ser148), which in turn is cleaved after Trp45 to yield the tyrosine-rich amelogenin polypeptide (Met1-Trp45) and the 13000 Da amelogenin (Leu46-Ser148) | ? | - |
? | |
additional information | Sus scrofa | recombinant Mmp-20 is self-activated by its incubation in 4-aminophenylmercuric acetate in 10 mM CaCl2 for 24 h | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-TRAP chelating nickel affinity column chromatography | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amelogenin + H2O | most of the secreted substrate is cleaved after Ser148 to generate the 20000 Da amelogenin (Met1-Ser148), which in turn is cleaved after Trp45 to yield the tyrosine-rich amelogenin polypeptide (Met1-Trp45) and the 13000 Da amelogenin (Leu46-Ser148) | Sus scrofa | ? | - |
? | |
additional information | recombinant Mmp-20 is self-activated by its incubation in 4-aminophenylmercuric acetate in 10 mM CaCl2 for 24 h | Sus scrofa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enamelysin | - |
Sus scrofa |
MMP-20 | - |
Sus scrofa |
General Information | Comment | Organism |
---|---|---|
physiological function | following the C-terminal cleavage of amelogenin by Mmp-20, co-assembly with its fragments leads to formation of nanorod structures whose properties eventually dictate the super-structural organization of enamel matrix, controlling the elongated growth of enamel apatite crystals | Sus scrofa |