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Literature summary for 3.4.24.B6 extracted from
- Amelogenin nanorods formation during proteolysis by Mmp-20 (2011), J. Struct. Biol., 176, 220-228.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1-Blue cells | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amelogenin + H2O | Sus scrofa | most of the secreted substrate is cleaved after Ser148 to generate the 20000 Da amelogenin (Met1-Ser148), which in turn is cleaved after Trp45 to yield the tyrosine-rich amelogenin polypeptide (Met1-Trp45) and the 13000 Da amelogenin (Leu46-Ser148) | ? | - |
? |  |
| additional information | Sus scrofa | recombinant Mmp-20 is self-activated by its incubation in 4-aminophenylmercuric acetate in 10 mM CaCl2 for 24 h | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-TRAP chelating nickel affinity column chromatography | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amelogenin + H2O | most of the secreted substrate is cleaved after Ser148 to generate the 20000 Da amelogenin (Met1-Ser148), which in turn is cleaved after Trp45 to yield the tyrosine-rich amelogenin polypeptide (Met1-Trp45) and the 13000 Da amelogenin (Leu46-Ser148) | Sus scrofa | ? | - |
? | |
| additional information | recombinant Mmp-20 is self-activated by its incubation in 4-aminophenylmercuric acetate in 10 mM CaCl2 for 24 h | Sus scrofa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enamelysin | - |
Sus scrofa |
| MMP-20 | - |
Sus scrofa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | following the C-terminal cleavage of amelogenin by Mmp-20, co-assembly with its fragments leads to formation of nanorod structures whose properties eventually dictate the super-structural organization of enamel matrix, controlling the elongated growth of enamel apatite crystals | Sus scrofa |
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