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Literature summary for 3.4.24.B6 extracted from

  • Lee, H.K.; Lee, D.S.; Ryoo, H.M.; Park, J.T.; Park, S.J.; Bae, H.S.; Cho, M.I.; Park, J.C.
    The odontogenic ameloblast-associated protein (ODAM) cooperates with RUNX2 and modulates enamel mineralization via regulation of MMP-20 (2010), J. Cell. Biochem., 111, 755-767.
    View publication on PubMed
Search for more literature for 3.4.24.B6

Cloned(Commentary)

Cloned (Comment) Organism
real-time PCR MMP-20 expression analysis, overview Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular MMP-20 is synthesized and secreted by ameloblasts Mus musculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amelogenin + H2O Mus musculus MMP-20 is involved in amelogenin processing and degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 newborn C57/Bl6J mice
-

Source Tissue

Source Tissue Comment Organism Textmining
ameloblast
-
 Mus musculus
-
enamel layer
-
 Mus musculus
-
odontoblast
-
 Mus musculus
-
tooth
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amelogenin + H2O
-
 Mus musculus ?
-
 ?
amelogenin + H2O MMP-20 is involved in amelogenin processing and degradation Mus musculus ?
-
 ?
casein + H2O zymography Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
MMP-20
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mus musculus

Expression

Organism Comment Expression
Mus musculus loss and siRNA-mediated silencing of Runx2 in ameloblast-lineage cells decreases ODAM expression, resulting in downregulation of MMP-20 expression down
Mus musculus increased expression of ODAM and Runx2 augments MMP-20 expression, and both TGF-beta1 and BMP-2 up-regulate Runx2 expression. TGF-beta1 promotes MMP-20 expression in ameloblasts up

General Information

General Information Comment Organism
physiological function MMP-20 is involved in the enamel formation and mineralization. The amelogenin degradation induced by MMP-20 is believed to be essential for the axial growth of enamel crystals MMP-20 activity is regulated by RUNX2, which is stimulated by BMP-2 or TGF-beta, and the odontogenic ameloblast-associated protein, ODAM, molecular mechanisms responsible for MMP-20 regulation, overview. Runx2 regulates ODAM expression, which in turn regulates MMP-20 expression and is recruited to the MMP-20 promoter. Increased MMP-20 expression accelerates amelogenin processing during enamel mineralization Mus musculus