Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, chromosome mapping to 11q22, expression in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tissue matrix metalloprotease inhibitor TIMP-2 | complete inhibition of amylogenin cleavage | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enzyme contains a zinc-binding site in the catalytic domain with the consensus sequence HEXGHXXGXXH at positions 226-236 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylogenin + H2O | Homo sapiens | involved in tooth formation | ? | - |
? | |
proteins + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60882 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | enzyme proform contains a prodomain motif PRCGVPD, the proform is in a latent state | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic degradation of ameloblastin | active site with the consensus sequence HEXGHXXGXXH at positions 226-236 | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from inclusion bodies expressed in Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
odontoblast | - |
Homo sapiens | - |
tooth | - |
Homo sapiens | - |
tooth enamel | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylogenin + H2O | involved in tooth formation | Homo sapiens | ? | - |
? | |
amylogenin + H2O | - |
Homo sapiens | 4 major peptide fragments of MWs 24 kDa, 23 kDa, 22 kDa, and 20 kDa | - |
? | |
Mca-PLA-norvaline-Dpa-AR-NH2 + H2O | synthetic fluorogenic substrate | Homo sapiens | ? | - |
? | |
Mca-PLGL-Dpa-AR-NH2 + H2O | synthetic fluorogenic substrate | Homo sapiens | ? | - |
? | |
proteins + H2O | - |
Homo sapiens | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enamelysin | - |
Homo sapiens |
M10.019 | Merops-ID | Homo sapiens |
MMP-20 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |