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Literature summary for 3.4.24.B5 extracted from
- Matrix-dependent proteolysis of surface transglutaminase by membrane-type metalloproteinase regulates cancer cell adhesion and locomotion (2001), J. Biol. Chem., 276, 18415-18422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of enzyme in HT1080 cells and U251 glioma cells, leading to enhanced levels of pro-matrix metalloprotease-2 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fibronectin | protects the cell surface tissue transglutaminase against degradation by MT1 MMP | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | on the cell surface | Homo sapiens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, MT1 MMP ectodomain, SDS-PAGE, x * 60000, MT1 MMP, active enzyme, SDS-PAGE | Homo sapiens |
| 60000 | - |
x * 42000, MT1 MMP ectodomain, SDS-PAGE, x * 60000, MT1 MMP, active enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell surface tissue transglutaminase + H2O | Homo sapiens | regulation of cancer cell adhesion, degradation supresses specifically cell adhesion and migration on fibronectin, but stimulates migration of cells on collagen | ? | - |
? |  |
| protein + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P50281 | membrane-type-1, -type -2 and -type-3 matrix metalloproteases | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HT-1080 cell | low expression level | Homo sapiens | - |
| U-251 cell | low expression level | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell surface tissue transglutaminase + H2O | at the leading edge of motile cancer cells, membrane-type-1, -type -2 and -type-3 matrix metalloproteases | Homo sapiens | 4 major and several minor cleavage products of cell surface tissue transglutaminase | - |
? | |
| cell surface tissue transglutaminase + H2O | regulation of cancer cell adhesion, degradation supresses specifically cell adhesion and migration on fibronectin, but stimulates migration of cells on collagen | Homo sapiens | ? | - |
? | |
| progelatinase A + H2O | substrate is activated by cleavage | Homo sapiens | gelatinase A + ? | - |
? | |
| protein + H2O | - |
Homo sapiens | peptides | - |
? | |
| protein + H2O | enzyme is involved in breakdown of extracellular matrix | Homo sapiens | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, MT1 MMP ectodomain, SDS-PAGE, x * 60000, MT1 MMP, active enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M10.015 | Merops-ID | Homo sapiens |
| membrane-type matrix metalloproteinase | - |
Homo sapiens |
| membrane-type-1 matrix metalloproteinase | - |
Homo sapiens |
| membrane-type-2 matrix metalloproteinase | - |
Homo sapiens |
| membrane-type-3 matrix metalloproteinase | - |
Homo sapiens |
| MT MMP | - |
Homo sapiens |
| MT1 MMP | - |
Homo sapiens |
| MT2 MMP | - |
Homo sapiens |
| MT3 MMP | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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