Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B4 extracted from

  • Li, C.H.; Cheng, Y.W.; Liao, P.L.; Yang, Y.T.; Kang, J.J.
    Chloramphenicol causes mitochondrial stress, decreases ATP biosynthesis, induces matrix metalloproteinase-13 expression, and solid-tumor cell invasion (2010), Toxicol. Sci., 116, 140-150.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B4

Inhibitors

Inhibitors Comment Organism Structure
CL-82198 a specific MMP-13 inhibitor, inhibits also the metastatic activity of chloramphenicol-treated cells Homo sapiens
additional information no inhibition of MMP-13 by MMP-3 inhibitor N-isobutyl-N-[4-methoxyphenylsulphonyl] glycyl hydroxamic acid or by doxycycline Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens
Zn2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
GBM-8401 cell
-
 Homo sapiens
-
Hep-G2 cell
-
 Homo sapiens
-
Huh-7 cell
-
 Homo sapiens
-
additional information no MMP-13 expression in THP-1 cells, HL-60 cells, and Jurkat cells Homo sapiens
-
NCI-H1299 cell
-
 Homo sapiens
-
NCI-N87 cell
-
 Homo sapiens
-
SAS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
collagenase-3
-
 Homo sapiens
MMP-13
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens both the JNK inhibitor SP 600125 and PI-3K/Akt inhibitor LY 294002 can inhibit chloramphenicol-induced c-Jun phosphorylation, MMP-13 expression, and cell invasion down
Homo sapiens chloramphenicol causes mitochondrial stress, decreases ATP biosynthesis, and induces MMP-13 expression, and solid-tumor cell invasion. Chloramphenicol also activated c-Jun N-terminal kinases, JNK, and PI-3K/Akt signaling, leading to c-Jun protein phosphorylation. The activated c-Jun protein activates binding to the MMP-13 promoter and also upregulates the amount of MMP-13. Other antibiotics that cause mitochondrial stress and a decrease in ATP biosynthesis also induce MMP-13 expression. Chloramphenicol-induction of MMP-13 expression is observed in nine solid-tumor cell lines, including H1299, HepG2, A549, CL-10, NCI-N87, Huh7, SAS, GBM8401, and Hep1, but not in leukemia suspensions, including THP-1, HL-60, and Jurkat cells up

General Information

General Information Comment Organism
additional information chloramphenicol-induced MMP-13 expression is strongly associated with metastatic capacity of solid-tumor cells Homo sapiens