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Literature summary for 3.4.24.B4 extracted from

  • Botos, I.; Meyer, E.; Swanson, S.M.; Lemaitre, V.; Eeckhout, Y.; Meyer, E.F.
    Structure of recombinant mouse collagenase-3 (MMP-13) (1999), J. Mol. Biol., 292, 837-844.
    View publication on PubMed
Search for more literature for 3.4.24.B4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3) Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme complexed with a hydroxamate inhibitor, hanging-drop vapour diffusion method, 17 mg/ml protein in 10 mM HEPES, pH 7.5, with ammonium sufate to saturation, 18°C, 60 days, X-ray diffraction structure determination at 2.0 A resolution, and analysis Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
hydroxamate inhibitor RS-113456 a diphenyl ether sulfon compound, complexing of the enzyme via His205 and Glu202 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ His201, His211, and His 205 are involved in metal ion binding, geometry from crystal structure Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Mus musculus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P33435 precursor
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Mus musculus

Reaction

Reaction Comment Organism Reaction ID
proteolytic degradation of proteins structure of the catalytic domain Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
chondrocyte hypertrophic, enzyme expression during bone development Mus musculus
-
osteoblast enzyme expression during bone development Mus musculus
-
periosteum enzyme expression during bone development Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O
-
 Mus musculus ?
-
 ?
cartilage + H2O
-
 Mus musculus ?
-
 ?
collagen type II + H2O
-
 Mus musculus ?
-
 ?
Fibronectin + H2O
-
 Mus musculus ?
-
 ?
protein + H2O
-
 Mus musculus peptides
-
 ?

Synonyms

Synonyms Comment Organism
M10.013 Merops-ID Mus musculus
matrix metalloproteinase-13
-
 Mus musculus
MMP-13
-
 Mus musculus
MMP13
-
 Mus musculus