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Literature summary for 3.4.24.B3 extracted from

  • Pan, W.; Arnone, M.; Kendall, M.; Grafstrom, R.H.; Seitz, S.P.; Wasserman, Z.R.; Albright, C.F.
    Identification of peptide substrates for human MMP-11 (stromelysin-3) using phage display (2003), J. Biol. Chem., 278, 27820-27827.
    View publication on PubMed
Search for more literature for 3.4.24.B3

Cloned(Commentary)

Cloned (Comment) Organism
expression of catalytic site comprising residues 98-272 and of mutants A235P and E216A in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
A235P mutant shows about 10fold increased activity with alpha1-proteinase inhibitor Homo sapiens
E216A active site mutant, catalytically inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
matrix metalloproteinase inhibitor AG3340 broad spectrum inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.148
-
 GGYAELRMGG pH 7.5, 37°C, recombinant mutant A235P Homo sapiens
0.207
-
 GGYAELRMGG pH 7.5, 37°C Homo sapiens
0.294
-
 GGAANLVRGG pH 7.5, 37°C, recombinant mutant A235P Homo sapiens
0.705
-
 GGAANLVRGG pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant catalytic domain and mutants from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
proteolytic degradation of proteins reaction and inhibition mechanism Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-antitrypsin inhibitor + H2O
-
 Homo sapiens ?
-
 ?
GAAGAMFLEA + H2O
-
 Homo sapiens GAAGA + MFLEA
-
 ?
GGAANLVRGG + H2O
-
 Homo sapiens GGAAN + LVRGG
-
 ?
GGPLALWARGG + H2O
-
 Homo sapiens GGPLA + LWARGG
-
 ?
GGPLGLYAGG + H2O
-
 Homo sapiens GGPLG + LYAGG
-
 ?
GGQPRGVWGG + H2O
-
 Homo sapiens GGQPRG + VWGG
-
 ?
GGTDAWLSGG + H2O
-
 Homo sapiens GGTDA + WLSGG
-
 ?
GGYAELRMGG + H2O best substrate Homo sapiens GGYAE + LRMGG
-
 ?
additional information wild-type enzyme does not cleave extracellular matrix proteins such as collagen, laminin, fibronectin, and elastin Homo sapiens ?
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?
protein + H2O substrate specificity, enzyme prefers 2 consensus sequences: 1. A(A/Q)(N/A)_(L/Y)(T/V/M/R)(R/K) and 2. G(G/A)E_LR, with _ marking the cleavage sites, enzyme also cleaves peptides containing Ala in position 3, Ala in position 1, and Leu/Tyr in position 1', peptides with proline in position 3 are poor substrates Homo sapiens peptides
-
 ?

Synonyms

Synonyms Comment Organism
M10.007 Merops-ID Homo sapiens
matrix metalloproteinase-11
-
 Homo sapiens
MMP-11 proteinase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.58
-
 GGYAELRMGG pH 7.5, 37°C, recombinant wild-type enzyme Homo sapiens
0.69
-
 GGAANLVRGG pH 7.5, 37°C, recombinant wild-type enzyme Homo sapiens
1.65
-
 GGAANLVRGG pH 7.5, 37°C, recombinant mutant A235P Homo sapiens
3 6 GGYAELRMGG pH 7.5, 37°C, recombinant mutant A235P Homo sapiens
3.08
-
 GGYAELRMGG pH 7.5, 37°C, recombinant mutant A235P Homo sapiens
6.08
-
 GGYAELRMGG pH 7.5, 37°C, recombinant wild-type enzyme Homo sapiens
6.08
-
 GGAANLVRGG pH 7.5, 37°C, recombinant wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.6 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000006
-
 matrix metalloproteinase inhibitor AG3340 pH 7.6, 37°C Homo sapiens