Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.B3 extracted from

  • Noel, A.; Santavicca, M.; Stoll, I.; L'Hoir, C.; Staub, A.; Murphy, G.; Rio, M.C.; Basset, P.
    Identification of structural determinants controlling human and mouse stromelysin-3 proteolytic activities (1995), J. Biol. Chem., 270, 22866-22872.
    View publication on PubMed
Search for more literature for 3.4.24.B3

Application

Application Comment Organism
pharmacology enzyme form with the specific residue A235 is an attractive target for the development of specific inhibitors for use in prevention of cancer progression Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of truncated mutant lacking the N-terminal prodomain in Escherichia coli BL21(DE3), and the C-terminal part of the hemopexin-like domain, expression of wild-type enzyme in MFC7 cells Mus musculus
expression of truncated mutant lacking the N-terminal prodomain, the C-terminal part of the hemopexin-like domain, and mutant A235P in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
A235P site-directed mutagenesis, alters substrate specificity, mutant processed and truncated enzyme additionally cleaves casein, laminin, and type IV collagen, Ala 235 is typical for the enzyme and is located immediately after the Met-turn, which forms a catalytic basis for the zinc ion Homo sapiens
P235A site-directed mutagenesis, truncated mutant with this introduces point mutation shows reduced enzyme activity against laminin and collgen IV Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloprotease, Zn2+ binding site is part of the catalytic centre Mus musculus
Zn2+ zinc binding region including the motif HEXXHXXGXXH and the conserved methionine residue of the Met-turn ALMX, Ala 235 is typical for the enzyme and is located immediately after the Met-turn, which forms a catalytic basis for the zinc ion Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, recombinant processed and truncated form, SDS-PAGE Mus musculus
22000
-
 x * 22000, recombinant processed and truncated form, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Mus musculus enzyme is involved in both physiological and pathological tissue remodeling processes, including those associated with cancer progression peptides
-
 ?
protein + H2O Homo sapiens enzyme is involved in both physiological and pathological tissue remodeling processes, including those associated with cancer progression peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification formation of a processed and truncated mature form of MW 21 kDa Mus musculus
proteolytic modification formation of a processed and truncated mature form of MW 22 kDa Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
mutant enzyme are solubilized and refolded from inclusion bodies after expression in Escherichia coli Homo sapiens
recombinant truncated enzyme is solubilized and refolded from inclusion bodies after expression in Escherichia coli Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
 recombinant processed and truncated enzyme, substrate beta-casein Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O only recombinant processed enzyme lacking the N-terminal prodomain and 175 amino acid residues of the C-terminus Mus musculus ?
-
 ?
casein + H2O only recombinant processed enzyme mutant A235P lacking the N-terminal prodomain and 175 amino acid residues of the C-terminus Homo sapiens ?
-
 ?
collagen type IV + H2O only recombinant processed enzyme lacking the N-terminal prodomain and 175 amino acid residues of the C-terminus Mus musculus ?
-
 ?
collagen type IV + H2O only recombinant processed enzyme mutant A235P lacking the N-terminal prodomain and 175 amino acid residues of the C-terminus Homo sapiens ?
-
 ?
Laminin + H2O only recombinant processed enzyme lacking the N-terminal prodomain and 175 amino acid residues of the C-terminus Mus musculus ?
-
 ?
Laminin + H2O only recombinant processed enzyme mutant A235P lacking the N-terminal prodomain and 175 amino acid residues of the C-terminus Homo sapiens ?
-
 ?
protein + H2O
-
 Mus musculus peptides
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?
protein + H2O enzyme is involved in both physiological and pathological tissue remodeling processes, including those associated with cancer progression Mus musculus peptides
-
 ?
protein + H2O enzyme is involved in both physiological and pathological tissue remodeling processes, including those associated with cancer progression Homo sapiens peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, recombinant processed and truncated form, SDS-PAGE Mus musculus
? x * 22000, recombinant processed and truncated form, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
M10.007 Merops-ID Mus musculus
M10.007 Merops-ID Homo sapiens
matrix metalloproteinase-11
-
 Mus musculus
matrix metalloproteinase-11
-
 Homo sapiens
matrixin
-
 Mus musculus
matrixin
-
 Homo sapiens
MMP-11
-
 Mus musculus
MMP-11
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Mus musculus
7.5
-
 assay at Homo sapiens