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Literature summary for 3.4.24.B2 extracted from

  • Chao, S.H.; Cheng, T.H.; Shaw, C.Y.; Lee, M.H.; Hsu, Y.H.; Tsai, Y.C.
    Characterization of a novel PepF-like oligopeptidase secreted by Bacillus amyloliquefaciens 23-7A (2006), Appl. Environ. Microbiol., 72, 968-971.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B2

Application

Application Comment Organism
additional information PepFBa can facilitate sporulation by processing a pro-Phr into active pentapeptides Bacillus amyloliquefaciens

Cloned(Commentary)

Cloned (Comment) Organism
from a Bacillus amyloliquefaciens 23-7A library Bacillus amyloliquefaciens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strongly inhibits at 10 mM Bacillus amyloliquefaciens
Cd2+ inhibits at 10 mM Bacillus amyloliquefaciens
Cu2+ inhibits at 10 mM Bacillus amyloliquefaciens
EDTA strongly inhibits at 10 mM, inhibition can by restored by Ca2+, Mg2+ and Co2+ Bacillus amyloliquefaciens
Fe2+ inhibits at 10 mM Bacillus amyloliquefaciens
additional information not inhibited by E-64, iodoacetamide, phosphoramidon and pehylmethylsulfonyl fluoride Bacillus amyloliquefaciens
Pb2+ inhibits at 10 mM Bacillus amyloliquefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0992
-
 N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala
-
 Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases activity Bacillus amyloliquefaciens
Mg2+ increases activity Bacillus amyloliquefaciens
Zn2+ zinc-binding motif, molar ratio of 1.87 Zn2+ to PepFBa Bacillus amyloliquefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 SDS-PAGE, purified enzyme Bacillus amyloliquefaciens
77050
-
 sequence analysis Bacillus amyloliquefaciens
170000
-
 gel filtration, native enzyme Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens Q71E77
-
-
Bacillus amyloliquefaciens 23-7A Q71E77
-
-

Purification (Commentary)

Purification (Comment) Organism
1035fold Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.7
-
 with N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala as substrate Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-phenylazobenzyloxycarbonyl-L-Pro-Leu-Gly-L-Pro-D-Arg + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
4-phenylazobenzyloxycarbonyl-L-Pro-Leu-Gly-L-Pro-D-Arg + H2O
-
 Bacillus amyloliquefaciens 23-7A ?
-
 ?
additional information no hydrolytic activities on bradykinin residues 1 to 5 Bacillus amyloliquefaciens ?
-
 ?
additional information no hydrolytic activities on bradykinin residues 1 to 5 Bacillus amyloliquefaciens 23-7A ?
-
 ?
N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
N-(3-(2-furyl)acryloyl)-Leu-Gly-Pro-Ala + H2O
-
 Bacillus amyloliquefaciens 23-7A ?
-
 ?
N-carboxylbenzoyl-Gly-Pro-Leu-Gly-Pro + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
N-carboxylbenzoyl-Gly-Pro-Leu-Gly-Pro + H2O
-
 Bacillus amyloliquefaciens 23-7A ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
PepFBa
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable for 1 h, thermally inactive at temperatures over 55°C Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus amyloliquefaciens