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Literature summary for 3.4.24.B2 extracted from

  • Monnet, V.; Nardi, M.; Chopin, A.; Chopin, M.C.; Gripon, J.C.
    Biochemical and genetic characterization of PepF, an oligopeptidase from Lactococcus lactis (1994), J. Biol. Chem., 269, 32070-32076.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pepF, DNA and amino acid sequence determination and analysis Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc binding site, HEXXH motif Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Lactococcus lactis
-
peptides
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis P54124 subsp. cremoris, i.e. Streptococcus cremoris, located on natural 55kb lactose-protease plasmid
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 75fold Lactococcus lactis

Reaction

Reaction Comment Organism Reaction ID
proteolytic cleavage of proteins at Leu-Gly cleavage of Gly-/-Xaa, enzyme often cleaves Phe-/-Xaa, with X being hydrophilic or basic, and with proline in position P'2 of the cleaved bond, no cleavage of bonds involving acidic residues Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.42
-
purified enzyme Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adrenocorticotropic hormone fragment 1-10 + H2O
-
Lactococcus lactis SYSMEHF + RWG
-
?
adrenocorticotropic hormone fragment 1-14 + H2O
-
Lactococcus lactis SYSMEHF + RWG + KPVG
-
?
adrenocorticotropic hormone fragment 1-17 + H2O
-
Lactococcus lactis SYSMEHF + RWG + KPV + GKKR
-
?
adrenocorticotropic hormone fragment 4-10 + H2O
-
Lactococcus lactis MEHF + RWG
-
?
beta-casomorphin + H2O
-
Lactococcus lactis YPFP + GPI
-
?
bradykinin + H2O best substrate Lactococcus lactis RPPGF + SPR
-
?
DesPro2bradykinin + H2O
-
Lactococcus lactis RPGF + SPFR
-
?
insulin chain B fragment 22-30 + H2O
-
Lactococcus lactis RGFF + Tyr + TPKA
-
?
Lys1bradykinin + H2O
-
Lactococcus lactis KPPGF + SPFR
-
?
additional information no activity with bradykinin fragment 2-10, Lys-p-nitroanilide, Gly-Pro-p-nitroanilide, adrenocorticotropic hormone fragment 1-4 SYSM and fragment 1-24, PQFY, Cbz-GPLGP, leucin enkephalin YGGFL, glucagon, full length insulin chain B, caseins Lactococcus lactis ?
-
?
neurotensin + H2O
-
Lactococcus lactis Pyr-LYENKPR + RPYIL
-
?
protein + H2O
-
Lactococcus lactis peptides
-
?
protein + H2O wide pH-dependent substrate specificity, cleaves peptides of length between 7 and 17 amino acids Lactococcus lactis peptides
-
?
substance P fragment 2-11 + H2O
-
Lactococcus lactis PKPQQ + Phe + Phe + GLM-NH2
-
?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
M03.007 Merops-ID Lactococcus lactis
PepF
-
Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
substrate specificty is pH-dependent Lactococcus lactis