Cloned (Comment) | Organism |
---|---|
gene pepF, DNA and amino acid sequence determination and analysis | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc binding site, HEXXH motif | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Lactococcus lactis | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | P54124 | subsp. cremoris, i.e. Streptococcus cremoris, located on natural 55kb lactose-protease plasmid | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, 75fold | Lactococcus lactis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolytic cleavage of proteins at Leu-Gly | cleavage of Gly-/-Xaa, enzyme often cleaves Phe-/-Xaa, with X being hydrophilic or basic, and with proline in position P'2 of the cleaved bond, no cleavage of bonds involving acidic residues | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
22.42 | - |
purified enzyme | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adrenocorticotropic hormone fragment 1-10 + H2O | - |
Lactococcus lactis | SYSMEHF + RWG | - |
? | |
adrenocorticotropic hormone fragment 1-14 + H2O | - |
Lactococcus lactis | SYSMEHF + RWG + KPVG | - |
? | |
adrenocorticotropic hormone fragment 1-17 + H2O | - |
Lactococcus lactis | SYSMEHF + RWG + KPV + GKKR | - |
? | |
adrenocorticotropic hormone fragment 4-10 + H2O | - |
Lactococcus lactis | MEHF + RWG | - |
? | |
beta-casomorphin + H2O | - |
Lactococcus lactis | YPFP + GPI | - |
? | |
bradykinin + H2O | best substrate | Lactococcus lactis | RPPGF + SPR | - |
? | |
DesPro2bradykinin + H2O | - |
Lactococcus lactis | RPGF + SPFR | - |
? | |
insulin chain B fragment 22-30 + H2O | - |
Lactococcus lactis | RGFF + Tyr + TPKA | - |
? | |
Lys1bradykinin + H2O | - |
Lactococcus lactis | KPPGF + SPFR | - |
? | |
additional information | no activity with bradykinin fragment 2-10, Lys-p-nitroanilide, Gly-Pro-p-nitroanilide, adrenocorticotropic hormone fragment 1-4 SYSM and fragment 1-24, PQFY, Cbz-GPLGP, leucin enkephalin YGGFL, glucagon, full length insulin chain B, caseins | Lactococcus lactis | ? | - |
? | |
neurotensin + H2O | - |
Lactococcus lactis | Pyr-LYENKPR + RPYIL | - |
? | |
protein + H2O | - |
Lactococcus lactis | peptides | - |
? | |
protein + H2O | wide pH-dependent substrate specificity, cleaves peptides of length between 7 and 17 amino acids | Lactococcus lactis | peptides | - |
? | |
substance P fragment 2-11 + H2O | - |
Lactococcus lactis | PKPQQ + Phe + Phe + GLM-NH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
M03.007 | Merops-ID | Lactococcus lactis |
PepF | - |
Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificty is pH-dependent | Lactococcus lactis |