Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme in Escherichia coli strain DH5alpha, expression of deletion mutant in Escherichia coli strain BMH 71-18 mut S | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of inactive mutant by site-directed mutagenesis in the ATP and Zn2+ binding domains, inactivation of the enzyme causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | - |
Saccharomyces cerevisiae | |
proteinase K | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Saccharomyces cerevisiae | 5743 | - |
mitochondrion | tightly associated with the inner mitochondrial membrane, oriented with the bulk of the protein facing the matrix | Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on, 2 zinc ligands are the conserved His540 and His546 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
unassembled gamma subunit of mitochondrial ATP-synthase + H2O | Saccharomyces cerevisiae | i.e. Atp3p | ? | - |
? | |
unassembled subunit II of cytochrome oxidase + H2O | Saccharomyces cerevisiae | i.e. Cox2p | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
unassembled gamma subunit of mitochondrial ATP-synthase + H2O | i.e. Atp3p | Saccharomyces cerevisiae | ? | - |
? | |
unassembled subunit II of cytochrome oxidase + H2O | i.e. Cox2p | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAA protease | - |
Saccharomyces cerevisiae |
M41.004 | Merops-ID | Saccharomyces cerevisiae |
Yme1p | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | required, contains ATP-binding site spanning from residues 316 to 328, GVLLTGPPGTGKT | Saccharomyces cerevisiae |