Literature summary for 3.4.24.B19 extracted from

Leonhard, K.; Herrmann, J.M.; Stuart, R.A.; Mannhaupt, G.; Neupert, W.; Langer, T.
AAA proteases with catalytic sites on opposite membrane surfaces comprise a proteolytic system for the ATP-dependent degradation of inner membrane proteins in mitochondria (1996), EMBO J., 15, 4218-4229.

Search for more literature for 3.4.24.B19

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant E41Q in deficient mutant cell DELTAyme1, complementation only by the wild-type	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
E541Q	site-directed mutagenesis, exchange in the active site, no complementation of deficient mutant cell DELTAyme1	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	strong	Saccharomyces cerevisiae
o-phenanthroline	strong	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrial inner membrane	integral, a large domain is exposed to the intermembrane space	Saccharomyces cerevisiae	5743	-
mitochondrion	-	Saccharomyces cerevisiae	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protein + H2O	Saccharomyces cerevisiae	proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	in complex with m-AAA protease Yta12p, EC 3.4.24.B18, complex is termed i-AAA protease	-

Reaction

Reaction	Comment	Organism	Reaction ID
proteolytic degradation of proteins	conserved metal-binding site HEXXH, selective degradation, mechanism	Saccharomyces cerevisiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hybrid protein of subunit 2 of cytochrome oxidase residues 1-74, mouse dihydrofolate reductase, and mitochondrial presequence, residues 1-66, of subunit 9 of the ATPase of Neurospora crassa + H2O	in vitro import into the mitochondrion	Saccharomyces cerevisiae	4 peptide fragments f1-f4	product characterization	?
protein + H2O	proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis	Saccharomyces cerevisiae	peptides	-	?
residues 1-74 of subunit 2 of cytochrome oxidase + H2O	two-step procedure, in vitro import into the mitochondrion	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
AAA protease	-	Saccharomyces cerevisiae
M41.004	Merops-ID	Saccharomyces cerevisiae
YME1	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)