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Literature summary for 3.4.24.B17 extracted from

  • Okuno, T.; Yamanaka, K.; Ogura, T.
    Characterization of mutants of the Escherichia coli AAA protease, FtsH, carrying a mutation in the central pore region (2006), J. Struct. Biol., 156, 109-114.
    View publication on PubMed
Search for more literature for 3.4.24.B17

Protein Variants

Protein Variants Comment Organism
C229F ATPase activity is 114% of wild-type activity, protease activity with BODIPY-casein is 47% of wild-type activity, protease activity with Cy3-sigma32 is 22% of wild-type activity Escherichia coli
D223K ATPase activity is 20% of wild-type activity, protease activity with BODIPY-casein is 10% of wild-type activity, protease activity with Cy3-sigma32 is 5% of wild-type activity Escherichia coli
D272N ATPase activity is 97% of wild-type activity, protease activity with BODIPY-casein is 81% of wild-type activity Escherichia coli
E226A ATPase activity is 10% of wild-type activity, protease activity with BODIPY-casein is 11% of wild-type activity Escherichia coli
E226K ATPase activity is less than 5% of wild-type activity, protease activity with BODIPY-casein is 6% of wild-type activity Escherichia coli
E226Q ATPase activity is 20% of wild-type activity, protease activity with BODIPY-casein is 18% of wild-type activity, protease activity with Cy3-sigma32 is 17% of wild-type activity Escherichia coli
E273A ATPase activity is 20% of wild-type activity, protease activity with BODIPY-casein is 8% of wild-type activity, protease activity with Cy3-sigma32 is less than 5% of wild-type activity Escherichia coli
E273D ATPase activity is 143% of wild-type activity, protease activity with BODIPY-casein is 82% of wild-type activity Escherichia coli
E273K ATPase activity is less 5% of wild-type activity, protease activity with BODIPY-casein is 7% of wild-type activity Escherichia coli
E273Q ATPase activity is 68% of wild-type activity, protease activity with BODIPY-casein is 50% of wild-type activity, protease activity with Cy3-sigma32 is 11% of wild-type activity Escherichia coli
H271D ATPase activity is 78% of wild-type activity, protease activity with BODIPY-casein is 39% of wild-type activity, protease activity with Cy3-sigma32 is 20% of wild-type activity Escherichia coli
M227K ATPase activity is 55% of wild-type activity, protease activity with BODIPY-casein is 69% of wild-type activity, protease activity with Cy3-sigma32 is 8% of wild-type activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AAI3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
BODIPY-casein + H2O
-
 Escherichia coli ?
-
 ?
LpxC + H2O
-
 Escherichia coli ?
-
 ?
sigma32 + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
FtsH
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP certain acidic residues in both the pore-1 and pore-2 loops are important for ATPase activity Escherichia coli