Literature summary for 3.4.24.B17 extracted from

Krzywda, S.; Brzozowski, A.M.; Karata, K.; Ogura, T.; Wilkinson, A.J.
Crystallization of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli (2002), Acta Crystallogr. Sect. D, 58, 1066-1067.

Search for more literature for 3.4.24.B17

Cloned(Commentary)

Cloned (Comment)	Organism
expression of AAA domain residues 126-398 as tagged protein in strain AR5088	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour-diffusion method, AAA domain solution: 10 mg/ml protein, 20 mM Tris-HCl, pH 8.5, 50 mM NaCl, 1 mM DTT, precipitant is 1.5 M ammonium sulfate, 2 to 3 days, X-ray structure determination and analysis at about 1.5 A	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protein + H2O	Escherichia coli	-	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant tagged AAA-domain, residues 126-398	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protein + H2O	-	Escherichia coli	peptides	-	?

Synonyms

Synonyms	Comment	Organism
FtsH	-	Escherichia coli
M41.001	Merops-ID	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	dependent on	Escherichia coli

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Release 2023.1 (January 2023)