Application | Comment | Organism |
---|---|---|
degradation | two-stage enzymatic reaction for the continuous measurement of LasA protease activity using a defined substrate, supplemented with Streptomyces griseus aminopeptidase for further cleavage of the product, rate of release of the chromophore can be measured spectrophotometrically | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
succinyl-Gly-Gly-Phe-4-nitroanilide | - |
Pseudomonas aeruginosa | |
11.2 | - |
succinyl-Gly-Gly-Leu-4-nitroanilide | - |
Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa FRD2128(pJKGT107) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-Gly-Gly-Leu-4-nitroanilide + H2O | - |
Pseudomonas aeruginosa | Leu-4-nitroanilide + succinyl-Gly-Gly | - |
? | |
succinyl-Gly-Gly-Leu-4-nitroanilide + H2O | - |
Pseudomonas aeruginosa FRD2128(pJKGT107) | Leu-4-nitroanilide + succinyl-Gly-Gly | - |
? | |
succinyl-Gly-Gly-Phe-4-nitroanilide + H2O | better substrate than succinyl-Gly-Gly-Leu-4-nitroanilide | Pseudomonas aeruginosa | Phe-4 nitroanilide + succinyl-Gly-Gly | - |
? | |
succinyl-Gly-Gly-Phe-4-nitroanilide + H2O | better substrate than succinyl-Gly-Gly-Leu-4-nitroanilide | Pseudomonas aeruginosa FRD2128(pJKGT107) | Phe-4 nitroanilide + succinyl-Gly-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LasA protease | - |
Pseudomonas aeruginosa |
staphylolysin | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.55 | - |
succinyl-Gly-Gly-Leu-4-nitroanilide | - |
Pseudomonas aeruginosa | |
11.8 | - |
succinyl-Gly-Gly-Phe-4-nitroanilide | - |
Pseudomonas aeruginosa |