Literature summary for 3.4.24.B15 extracted from

Barros, N.M.; Nascimento, F.D.; Oliveira, V.; Juliano, M.A.; Juliano, L.; Loisel, T.; Nader, H.B.; Boileau, G.; Tersariol, I.L.; Carmona, A.K.
The critical interaction of the metallopeptidase PHEX with heparan sulfate proteoglycans (2008), Int. J. Biochem. Cell Biol., 40, 2781-2792.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in CHO cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
chondroitin sulfate	-	Homo sapiens
EDTA	5 mM, complete inhibition	Homo sapiens
heparan sulfate	-	Homo sapiens
heparin	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Homo sapiens	5783	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	glycosaminoglycans interact with PHEX, interfering with its enzyme activity, protein stability and cellular trafficking. This interaction may regulate PHEX function influencing the mineralization process	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
osteoblast	PHEX colocalizes with heparan sulfate at the cell surface of osteoblasts	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-aminobenzoyl-Gly-Phe-Ser-Asp-Tyr-Lys(Dnp)-OH + H2O	-	Homo sapiens	2-aminobenzoyl-Gly-Phe-Ser + Asp-Tyr-Lys(Dnp)-OH	-	?
additional information	glycosaminoglycans interact with PHEX, interfering with its enzyme activity, protein stability and cellular trafficking. This interaction may regulate PHEX function influencing the mineralization process	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
PHEX	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	PHEX is protected by glycosaminoglycans against thermal denaturation. The presence of heparin stabilizes the overall PHEX tertiary structure against denaturation at high temperature	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000025	-	heparin	-	Homo sapiens
0.000306	-	heparan sulfate	-	Homo sapiens
0.000869	-	chondroitin sulfate	-	Homo sapiens

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Release 2023.1 (January 2023)