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Literature summary for 3.4.24.B15 extracted from

  • Guo, R.; Rowe, P.S.; Liu, S.; Simpson, L.G.; Xiao, Z.S.; Darryl Quarles, L.D.
    Inhibition of MEPE cleavage by Phex (2002), Biochem. Biophys. Res. Commun., 297, 38-45.
    View publication on PubMed
Search for more literature for 3.4.24.B15

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzyme in Spodoptera frugiperda Sf9 cells via baculovirus infection, recombinant enzyme is located in the membranes of the Sf9 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of an inactive C-terminal deletion mutant of the enzyme Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibits proteolyic activity but not the protease inhibiting activity of the enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Mus musculus inactivating mutations cause X-linked hypophosphatemia, i.e. XLH, which results in the local accumulation of an unknown autocrine/paracrine factor in bone that inhibits mineralization of extracellular matrix peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant enzyme, but not the C-terminal deletion mutant, inhibited the cleavage of recombinant human matrix phosphoglycoprotein MEPE by endogenous cathepsin-like enzyme activity in SF9 insect cells, MEPE is no substrate for PHEX Mus musculus ?
-
 ?
protein + H2O
-
 Mus musculus peptides
-
 ?
protein + H2O inactivating mutations cause X-linked hypophosphatemia, i.e. XLH, which results in the local accumulation of an unknown autocrine/paracrine factor in bone that inhibits mineralization of extracellular matrix Mus musculus peptides
-
 ?
ZAAL-4-nitroanilide + H2O recombinant wild-type enzyme, not the recombinant mutant Mus musculus Z-Ala-Ala-Leu + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
M13.091 Merops-ID Mus musculus
PHEX
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Mus musculus