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Literature summary for 3.4.24.B15 extracted from
- Analysis of recombinant Phex: an endopeptidase in search of a substrate (2001), Am. J. Physiol., 281, E837-847.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA determination and analysis, expression of wild-type and C-terminally truncated mutant as His-tagged protein in Spodoptera frugiperda Sf9 cells via baculovirus infection | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of C-terminally truncated enzyme version | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | type II membrane-bound zinc metalloprotease | Mus musculus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | zinc metalloprotease | Homo sapiens |  |
| Zinc | type II membrane-bound zinc metalloprotease | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
| 86000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
| 100000 | - |
x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Mus musculus | - |
peptides | - |
? | |
| protein + H2O | Homo sapiens | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
type II membrane-bound zinc metalloprotease | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 6 N-glycosylation sites, recombinant and wild-type enzyme | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from SF9 insect cells as His-tagged protein | Mus musculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| proteolytic degradation of proteins | enzyme is an endopeptidase | Homo sapiens | |
| proteolytic degradation of proteins | enzyme is an endopeptidase | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| MC3T3-E1 cell | osteoblast cell culture | Mus musculus | - |
| MG-63 cell | osteoblast cell culture | Homo sapiens | - |
| osteoblast | - |
Mus musculus | - |
| osteoblast | cell culture | Homo sapiens | - |
| SAOS-2 cell | osteoblast cell culture | Homo sapiens | - |
| U2-OS cell | osteoblast cell culture | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | human stanniocalcin STC-1 is no substrate, recombinant rat parathyroid hormone is no substrate for the wild-type and mutant recombinant enzyme | Mus musculus | ? | - |
? | |
| additional information | human stanniocalcin STC-1, casein and wild-type as well as mutant FGF-23 proteins are no substrates, recombinant rat parathyroid hormone is no substrate for the wild-type and mutant recombinant enzyme | Homo sapiens | ? | - |
? | |
| protein + H2O | - |
Mus musculus | peptides | - |
? | |
| protein + H2O | - |
Homo sapiens | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant C-terminally truncated mutant, SDS-PAGE, x * 86000, deglycosylated recombinant enzyme, SDS-PAGE, x * 100000, glycosylated recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M13.091 | Merops-ID | Mus musculus |
| M13.091 | Merops-ID | Homo sapiens |
| PHEX | - |
Mus musculus |
| PHEX | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Mus musculus |
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