Inhibitors | Comment | Organism | Structure |
---|---|---|---|
angiotensin | - |
Rattus norvegicus | |
bradykinin | - |
Rattus norvegicus | |
cholecystokinin(1-6) | - |
Rattus norvegicus | |
cholecystokinin-8 | - |
Rattus norvegicus | |
Leu-Met | - |
Rattus norvegicus | |
Leu-Met-NH2 | - |
Rattus norvegicus | |
Leu5-enkephalin | - |
Rattus norvegicus | |
Leu5-enkephalin-NH2 | - |
Rattus norvegicus | |
Substance P | - |
Rattus norvegicus | |
Val-Phe | - |
Rattus norvegicus | |
Val-Phe-NH2 | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme L739G | Rattus norvegicus | |
0.044 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme L739G/S133G | Rattus norvegicus | |
0.045 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme R131M | Rattus norvegicus | |
0.062 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2. wild-type enzyme | Rattus norvegicus | |
0.185 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme S133G | Rattus norvegicus | |
1 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme N567G | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin + H2O | cleavage sites: DRVY-/-IHP-/-FHL | Rattus norvegicus | ? | - |
? | |
bradykinin + H2O | cleavage sites: RPPGFSP-/-FR | Rattus norvegicus | ? | - |
? | |
cholecystokinin(1-6) + H2O | cleavage site: DYMG-/-WM | Rattus norvegicus | ? | - |
? | |
cholecystokinin-8 + H2O | cleavage site: DYMG-/-WMDF-NH2 | Rattus norvegicus | ? | - |
? | |
Leu5-enkephalin + H2O | cleavage site: YGG-/-FL | Rattus norvegicus | ? | - |
? | |
Leu5-enkephalin-NH2 + H2O | cleavage site: YGG-/-FL-NH2 | Rattus norvegicus | ? | - |
? | |
Substance P + H2O | cleavage sites: RPKPQQ-/-FFG-/-LM-NH2 | Rattus norvegicus | ? | - |
? | |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O | - |
Rattus norvegicus | succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NEP2 | - |
Rattus norvegicus |
neprilysin 2 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
1.97 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme S133G | Rattus norvegicus | |
5.23 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme N567G | Rattus norvegicus | |
14.03 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
17.3 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme R131M | Rattus norvegicus | |
19 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme L739G/S133G | Rattus norvegicus | |
30.7 | - |
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide | 37°C, pH 7.2, mutant enzyme L739G | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Leu5-enkephalin | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.003 | - |
bradykinin | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.008 | - |
angiotensin | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.01 | - |
cholecystokinin-8 | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.011 | - |
Substance P | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.02 | - |
Leu-Met | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.02 | - |
Leu5-enkephalin-NH2 | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.021 | - |
Val-Phe | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.05 | - |
cholecystokinin(1-6) | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.13 | - |
Val-Phe-NH2 | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus | |
0.175 | - |
Leu-Met-NH2 | 37°C, pH 7.2, wild-type enzyme | Rattus norvegicus |