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Literature summary for 3.4.24.B14 extracted from

  • Voisin, S.; Ouimet, T.
    The ultimate tryptophan residue of neprilysin 2 is not involved in protein maturation and enzymatic activity (2005), Biochem. Biophys. Res. Commun., 335, 356-360.
    View publication on PubMed
Search for more literature for 3.4.24.B14

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutated forms of rNEP2 in AtT20 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
P751A no hydrolysis of succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide Rattus norvegicus
P764Y kcat/Km is 2.6fold lower than wild-type enzyme Rattus norvegicus
W774A kcat/Km is 1.1fold lower than wild-type enzyme Rattus norvegicus
W774F kcat/Km is nearly identical to wild-type value Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, wild-type enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide
-
 ?

Synonyms

Synonyms Comment Organism
neprilysin 2
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme W774A Rattus norvegicus
3.5
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme P764Y Rattus norvegicus
7.8
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme W774F Rattus norvegicus
8.7
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, wild-type enzyme Rattus norvegicus