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Literature summary for 3.4.24.B12 extracted from

  • Miwa, H.E.; Gerken, T.A.; Huynh, T.D.; Duesler, L.R.; Cotter, M.; Hering, T.M.
    Conserved sequence in the aggrecan interglobular domain modulates cleavage by ADAMTS-4 and ADAMTS-5 (2009), Biochim. Biophys. Acta, 1790, 161-172.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B12

Inhibitors

Inhibitors Comment Organism Structure
additional information substrate specificity of ADAMTS-5 against recombinant aggrecan, aggrecan mutants S377Q and S377T lead to aggrecan cleavage inhibition Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens
-
 recombinant
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aggrecan + H2O 2 microg, 90 min, reaction stopped by addition of 21 mM EDTA Homo sapiens ?
-
 ?
aggrecan + H2O recombinant bovine aggrecan mutated at amino acids N-terminal or C-terminal to the interglobular domain cleavage site. Identification of multiple conserved amino acids within regions N- and C-terminal to the site of scission that may influence enzyme-substrate recognition, and may interact with exosites on ADAMTS-5. The S377Q mutant is extremely resistant to cleavage at Glu373-Ala374 by ADAMTS-5. Cleavage of S377T with ADAMTS-5 is also significantly inhibited compared to wild-type. The other P4'-mutant digests are similar to wild-type. Gln or Thr at the P4' position are inhibitory to ADAMTS-5 cleavage Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ADAMTS-5
-
 Homo sapiens