Cloned (Comment) | Organism |
---|---|
adamts-1 gene maps to chromosome 16, region C3-C5, transient expression in COS-7 cells of FLAG-tagged wild-type and deletion mutants and mutant E386Q | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
E386Q | oligonucleotide primer mutagenesis, mutation within the zinc binding motif, loss of zinc-binding capacity, loss of activity and capacity to complex with alpha2-macroglobulin | Mus musculus |
additional information | construction of soluble C-terminal deletion mutants, that are still able to form complexes with alpha2-macroglobulin | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition of complex formation with alpha2-macroglobulin | Mus musculus | |
additional information | no inhibition by leupeptin, aprotinin, phenylmethylsulfonyl floride, and pepstatin | Mus musculus | |
Phenanthroline | inhibition of complex formation with alpha2-macroglobulin | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted protein binds to the matrix | Mus musculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloprotease, contains zinc binding motif HEXXHXXXXXHD in the metalloprotease domain | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
proteins + H2O | Mus musculus | enzyme is involved in the inflammatory process | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | processing by furin endopeptidase in vivo | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha2-macroglobulin + H2O | cleavage of the bait region of alpha2-macroglobulin, complex formation with alpha2-macroglobulin is dependent on the proteolytic activity | Mus musculus | ? | - |
? | |
proteins + H2O | - |
Mus musculus | peptides | - |
? | |
proteins + H2O | enzyme is involved in the inflammatory process | Mus musculus | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADAMTS-1 | - |
Mus musculus |
M12.222 | Merops-ID | Mus musculus |