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Literature summary for 3.4.24.B11 extracted from
- ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan (2000), FEBS Lett., 478, 241-245.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| transient expression in HEK293 cells of FLAG- and His-tagged wild-type and deletion mutants, the majority of the recombinant protein is expressed in an active form without the proprotein domain | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E386Q | oligonucleotide primer mutagenesis, mutation within the zinc binding motif, inactive | Mus musculus |
| additional information | construction of soluble C-terminal deletion mutants | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Mus musculus |  |
| additional information | no inhibition by aprotinin and leupeptin | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | matrix | Mus musculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | metalloproteinase | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aggrecan + H2O | Mus musculus | substrate is a cartilage proteoglycan | ? | - |
? | |
| proteins + H2O | Mus musculus | - |
peptides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutants | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cartilage | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aggrecan + H2O | C-terminal spacer region of the enzyme is required for activity, substrate is a cartilage proteoglycan, cleavage site is the peptide bond Glu1871-Leu1872 within the substrates chondroitin sulfate attachment domain | Mus musculus | ? | - |
? | |
| aggrecan + H2O | substrate is a cartilage proteoglycan | Mus musculus | ? | - |
? | |
| proteins + H2O | - |
Mus musculus | peptides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| a disintegrin and metalloproteinase with thrombospondin motifs 1 | - |
Mus musculus |
| ADAMTS-1 | - |
Mus musculus |
| M12.222 | Merops-ID | Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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