Cloned (Comment) | Organism |
---|---|
transient expression in COS-7 cells of an enzyme mutant where the signal peptide, prodomain and metalloprotease domain are exchanged for an Ig kappa-chain leader sequence, secretion to the medium | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C179A | site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain results in a no longer latent but inactive zymogen | Homo sapiens |
C179S | site-directed mutagenesis, exchange of the cysteine-switch residue of the prodomain results in a no longer latent but inactive zymogen | Homo sapiens |
E351Q | site-directed mutagenesis, exchange of the glutamic acid residue of the zinc binding domain involved in catalytic activity, inactive to form complexes with alpha2-macroglobulin | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
EDTA | complete inhibition | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | highly conserved zinc-binding motif HEXXHXXGXXH, the metalloprotease domain is the catalytically active site, the Met-turn is essential for the structural integrity of the zinc-binding site, Glu351 is essential for activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, processed recombinant mutant enzyme, SDS-PAGE, x * 92000, unprocessed recombinant mutant enzyme, SDS-PAGE | Homo sapiens |
92000 | - |
x * 68000, processed recombinant mutant enzyme, SDS-PAGE, x * 92000, unprocessed recombinant mutant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme complexes alpha2-macroglobulin | ? | - |
? | |
proteins + H2O | Homo sapiens | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43184 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the cysteine-switch of the prodomain maintaines the zymogen in a latent state, enzyme is synthesized as a catalytically inactive zymogen, in vivo cleavage by a furin-like endopeptidase, activation is chemically possible with N-ethylmaleimide, but not with Hg2+ | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
proteolysis of proteins | the metalloprotease domain is catalytically active, Glu351 is essential for activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme complexes alpha2-macroglobulin | Homo sapiens | ? | - |
? | |
protein + H2O | - |
Homo sapiens | peptides | - |
? | |
proteins + H2O | - |
Homo sapiens | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, processed recombinant mutant enzyme, SDS-PAGE, x * 92000, unprocessed recombinant mutant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
a disintegrin and metalloproteinase domain 12 | - |
Homo sapiens |
ADAM12 | - |
Homo sapiens |
M12.212 | Merops-ID | Homo sapiens |
meltrin alpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
alpha2-macroglobulin complexing assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
alpha2-macroglobulin complexing assay at | Homo sapiens |